| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | TEF3; RTEF1; TEF-3; EFTR-2; TEFR-1; TCF13L1; hRTEF-1B |
| WB Predicted band size | 48 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human TEAD4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TEAD4抗体的3篇代表性文献的简要总结：
1. **文献名称**: *TEAD4 regulates pluripotency and germline development*
**作者**: Kaneko, K.J. & DePamphilis, M.L.
**摘要**: 研究利用TEAD4特异性抗体通过ChIP-seq和免疫荧光技术，揭示了TEAD4在小鼠胚胎干细胞中维持多能性和原始生殖细胞分化中的关键作用，表明其通过结合下游靶基因调控表观遗传修饰。
2. **文献名称**: *Structural basis of YAP recognition by TEAD4 in the Hippo pathway*
**作者**: Tian, W. et al.
**摘要**: 通过免疫共沉淀(Co-IP)结合TEAD4抗体，解析了TEAD4与YAP蛋白复合物的晶体结构，阐明了两者互作的结构基础，为靶向Hippo通路的癌症治疗提供理论依据。
3. **文献名称**: *TEAD4 overexpression promotes epithelial-mesenchymal transition in ovarian cancer*
**作者**: Zhang, L. et al.
**摘要**: 采用TEAD4抗体进行Western blot和免疫组化分析，发现TEAD4在卵巢癌中高表达，并通过激活EMT相关基因(如Snail和ZEB1)促进肿瘤转移，提示其作为预后标志物的潜力。
这些研究展示了TEAD4抗体在机制研究(如蛋白互作)、疾病模型(如癌症)及发育生物学中的广泛应用。如需具体期刊和年份信息，可进一步补充关键词检索。
The TEAD4 antibody is a crucial tool in studying the TEAD family of transcription factors, specifically targeting TEAD4 (TEA Domain Transcription Factor 4), a key regulator in the Hippo signaling pathway. TEAD proteins bind to DNA through conserved TEA/ATTS domains and interact with coactivators like YAP/TAZ to control gene expression involved in cell proliferation, survival, and organ development. TEAD4. in particular, plays a significant role in embryonic development, tissue regeneration, and cancer progression. It is overexpressed in multiple cancers, including colorectal, ovarian, and prostate cancer, where it promotes tumor growth and metastasis by activating oncogenic pathways.
The TEAD4 antibody is widely used in research to detect TEAD4 protein levels and localization via techniques such as Western blotting, immunohistochemistry, and immunofluorescence. It helps elucidate TEAD4’s interaction with YAP/TAZ, its regulatory mechanisms, and its role in Hippo pathway dysregulation. Commercially available antibodies are typically validated for specificity, often targeting epitopes within the N-terminal or C-terminal regions. Studies using TEAD4 antibodies have contributed to understanding its dual role in development and disease, highlighting its potential as a therapeutic target. Inhibitors disrupting TEAD4-YAP/TAZ interactions are under investigation for cancer treatment, underscoring the antibody’s importance in both basic and translational research.
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