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产品描述：猪降钙素基因相关肽(CGRP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CGRP含量。CGRP（降钙素基因相关肽）是一种具有强大血管舒张活性的神经肽。背景上，其在偏头痛发病机制中起到重要作用。研究机制方面，它可通过与受体结合，介导神经源性炎症、扩张硬脑膜血管等，引发偏头痛相关症状，是偏头痛治疗的热门靶点。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于神经生物学、心血管研究、疼痛机制探索等科研领域，尤其适合用于建立动物模型（如偏头痛或高血压模型）后评估CGRP动态变化，或通过组织样本分析特定器官中CGRP的表达水平。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：CGRP
Uniprot No.：A6P7L6
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig CGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 84-94 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 96-102 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 94-102 | |||||
回收率：
| The recovery of pig CGRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 90-96 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.0282.2042.1161.93210001.5601.6871.6241.4405001.0491.0341.0420.8582500.6650.6470.6560.4721250.3770.3980.3880.20462.50.3120.3250.3190.13531.250.2360.2410.2390.05500.1820.1850.184 | ||||||
货期：3-5 working days
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