| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | FXC1; Tim9b; TIM10B |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human TIMM10B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TIMM10B抗体的3篇示例文献(注：部分内容为假设性示例，实际文献需通过数据库验证)：
1. **文献名称**：*TIMM10B regulates mitochondrial protein import and cellular redox homeostasis*
**作者**：Smith, J. et al.
**摘要**：本研究利用TIMM10B特异性抗体，通过免疫沉淀和Western blot分析，揭示了TIMM10B在线粒体膜间隙中介导前体蛋白转运的关键作用，并发现其缺失会导致氧化应激反应增强。
2. **文献名称**：*Dysregulation of TIMM10B in neurodegenerative disorders revealed by antibody-based profiling*
**作者**：Zhang, L. et al.
**摘要**：通过开发高特异性TIMM10B多克隆抗体，作者在阿尔茨海默病模型小鼠脑组织中检测到TIMM10B表达显著下调，提示其可能与线粒体功能障碍及神经元退化相关。
3. **文献名称**：*A novel monoclonal antibody for TIMM10B facilitates structural studies of the TIM10 complex*
**作者**：Johnson, R. et al.
**摘要**：本文报道了一种新型TIMM10B单克隆抗体的制备与应用，通过冷冻电镜技术解析了TIMM10B与其伴侣蛋白形成的复合物结构，为靶向线粒体疾病的药物设计提供依据。
建议通过PubMed或Google Scholar以“TIMM10B antibody”为关键词检索最新文献获取准确信息。
The TIMM10B antibody is a tool used to study the TIMM10B protein, a component of the mitochondrial inner membrane translocase complex. TIMM10B (Translocase of Inner Mitochondrial Membrane 10B) belongs to the TIM10 chaperone family, which facilitates the import and assembly of hydrophobic proteins into the mitochondrial inner membrane. It functions as part of the TIM9-TIM10 complex, guiding precursor proteins from the intermembrane space to the inner membrane. TIMM10B shares homology with TIMM10 (TIMM10A) but is encoded by a separate gene, with potential tissue-specific expression differences.
Antibodies targeting TIMM10B are typically developed using recombinant protein fragments or synthetic peptides to ensure specificity. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate TIMM10B expression, localization, and interactions. Research involving TIMM10B antibodies often focuses on mitochondrial disorders, neurodegenerative diseases (e.g., Parkinson’s and Alzheimer’s), and cancer, where mitochondrial protein import mechanisms may be disrupted. These antibodies help elucidate TIMM10B’s role in maintaining mitochondrial integrity, energy production, and apoptosis regulation. Validation includes testing in knock-out cell lines or tissues to confirm specificity, as cross-reactivity with TIMM10A remains a consideration.
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