| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| WB Predicted band size | 38 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human POLB |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于POLB(DNA聚合酶β)抗体的3篇参考文献摘要示例(文献信息为模拟，非真实存在)：
1. **文献名称**: "A highly specific monoclonal antibody for detecting DNA polymerase β in mammalian cells"
**作者**: Smith A, et al.
**摘要**: 该研究报道了一种新型单克隆抗体的开发，能够特异性识别哺乳动物细胞中的DNA聚合酶β(POLB)。通过Western blot和免疫荧光验证其特异性，证实该抗体在检测POLB表达水平及亚细胞定位中的应用价值，尤其在DNA损伤修复研究中的潜力。
2. **文献名称**: "DNA polymerase β deficiency and antibody-based detection in colorectal cancer"
**作者**: Lee C, et al.
**摘要**: 研究利用POLB抗体分析结直肠癌组织中POLB蛋白的表达缺失，发现POLB低表达与微卫星不稳定性(MSI)及化疗耐药性相关。通过免疫组化证实抗体在临床样本中的可靠性，为癌症生物标志物研究提供工具。
3. **文献名称**: "Characterization of DNA repair enzyme POLB in neurodegenerative models using custom polyclonal antibodies"
**作者**: Garcia R, et al.
**摘要**: 研究团队开发了兔源多克隆抗体，用于分析阿尔茨海默病模型中POLB的异常表达及功能。抗体验证显示其适用于脑组织切片和神经元细胞的POLB定量分析，揭示了POLB缺陷与神经元DNA损伤积累的关联。
(注：以上文献为示例，实际文献需通过PubMed/Google Scholar等平台检索关键词“POLB antibody”或“DNA polymerase beta antibody”获取。)
The POLB antibody targets DNA polymerase beta (Pol β), a key enzyme in the base excision repair (BER) pathway responsible for repairing small DNA lesions caused by oxidation, alkylation, or hydrolysis. Encoded by the POLB gene, this 39 kDa nuclear protein is critical for maintaining genomic stability by accurately filling single-strand gaps during DNA repair. Pol β's role extends beyond BER; it is implicated in somatic hypermutation and class-switch recombination in immune cells. Dysregulation or mutations in POLB are linked to cancer, neurodegenerative disorders, and chemotherapy resistance, making it a focus in oncology and disease research.
POLB antibodies are essential tools for detecting Pol β expression and localization in cells or tissues. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study DNA repair mechanisms, cellular responses to genotoxic stress, and POLB-related pathologies. Researchers also employ these antibodies to explore Pol β's functional interactions with other repair proteins or its potential as a therapeutic target. Commercial POLB antibodies are typically raised in rabbits or mice, validated for specificity via knockout controls, and optimized for cross-reactivity in human, mouse, or rat samples. Their applications span basic research, biomarker discovery, and drug development studies.
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