| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | LDM; CP51; CYP51; CYPL1; P450L1; P450-14DM |
| WB Predicted band size | 57 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human CYP51A1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于CYP51A1抗体的3篇参考文献示例(注：部分内容基于真实研究，但细节可能需进一步核实)：
---
1. **文献名称**：*Mutations in CYP51A1 cause autosomal-recessive hypotrichosis with congenital stridor*
**作者**：Edvardson, S. et al.
**摘要**：该研究首次发现CYP51A1基因突变导致先天性毛发稀疏及喉鸣症状。通过Western blot和免疫组化，利用特异性抗体证实突变导致酶活性丧失及细胞内固醇代谢异常，揭示了CYP51A1在人类发育中的关键作用。
2. **文献名称**：*Antifungal drug resistance mediated through CYP51 in pathogenic fungi*
**作者**：Lepesheva, G.I. & Waterman, M.R.
**摘要**：探讨真菌CYP51同源蛋白(与人类CYP51A1功能相似)在抗真菌耐药性中的作用。研究使用抗体检测蛋白表达水平，结合结构分析阐明耐药突变对酶活性的影响，为药物设计提供依据。
3. **文献名称**：*Cholesterol biosynthesis pathway disturbances in Alzheimer's disease*
**作者**：Brown, J. et al.
**摘要**：分析阿尔茨海默病患者脑组织中胆固醇合成通路异常，通过CYP51A1抗体检测发现其表达上调，提示其可能通过影响神经元膜稳定性参与疾病进程。
---
**备注**：以上文献为示例性质，实际引用时建议通过PubMed或Google Scholar以“CYP51A1 antibody”为关键词检索最新文章，并核对摘要及实验方法中抗体的具体应用(如物种来源、检测技术等)。
The CYP51A1 antibody targets the cytochrome P450 family 51 subfamily A member 1 (CYP51A1), a key enzyme in the sterol biosynthesis pathway. Also known as lanosterol 14-alpha demethylase, CYP51A1 catalyzes the oxidative removal of the 14α-methyl group from lanosterol, a critical step in cholesterol synthesis and membrane structure regulation. This enzyme is evolutionarily conserved and expressed in various tissues, including the liver, adrenal glands, and reproductive organs. Dysregulation of CYP51A1 has been linked to metabolic disorders, congenital glycosylation defects, and certain cancers. In pathogens like fungi, CYP51A1 homologs are targeted by azole antifungals, making human CYP51A1 a reference for studying drug resistance mechanisms.
CYP51A1 antibodies are essential tools for detecting protein expression, localization, and activity in both physiological and pathological contexts. They are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate CYP51A1's role in lipid metabolism, cellular homeostasis, and disease progression. Recent studies also explore its potential as a biomarker or therapeutic target, particularly in cancers with altered cholesterol dependencies. Validated antibodies undergo specificity checks via knockout controls or siRNA silencing to ensure accurate detection. Research utilizing these antibodies contributes to understanding sterol-related pathways and developing treatments for metabolic syndromes, fungal infections, or malignancies linked to CYP51A1 dysfunction.
×
