Rabbit Polyclonal ORC2 Antibody

The ORC2 antibody targets the Origin Recognition Complex subunit 2 (ORC2), a critical component of the six-subunit ORC complex essential for initiating DNA replication in eukaryotic cells. Discovered in yeast and conserved across eukaryotes, ORC binds to replication origins, serving as a scaffold to recruit CDC6. CDT1. and the MCM helicase complex during the G1 phase of the cell cycle. ORC2. along with ORC3-5. forms the core ATPase module, while ORC1 and ORC6 stabilize DNA interactions. Antibodies against ORC2 are widely used to study DNA replication mechanisms, cell cycle regulation, and chromatin organization. They enable detection of ORC2 localization (e.g., via immunofluorescence), quantification in cell lysates (Western blotting), and investigation of replication origin dynamics (ChIP-seq). Dysregulation of ORC2 has been implicated in cancers, such as colorectal and hepatocellular carcinoma, where overexpression may drive uncontrolled replication. Research using ORC2 antibodies has also explored developmental defects linked to replication errors and potential therapeutic targets. These antibodies are typically raised against epitopes in conserved regions (e.g., human ORC2 residues 1-300) and validated for specificity across models (human, mouse, rat). Their applications extend to probing protein interactions (co-immunoprecipitation with ORC subunits) and assessing replication stress responses in diseases.