| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | COXVB |
| WB Predicted band size | 14 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human COX5B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DDX24抗体的3篇文献示例(注：以下内容为模拟，实际文献需通过学术数据库验证):
1. **文献名称**: *DDX24 regulates antiviral innate immunity through NLRP3 inflammasome activation*
**作者**: Li, X. et al.
**摘要**: 研究揭示了DDX24通过调控NLRP3炎症小体在抗病毒天然免疫中的作用，利用DDX24抗体进行免疫共沉淀和Western blot实验，证实其与NLRP3的相互作用。
2. **文献名称**: *Oncogenic role of DEAD-box helicase DDX24 in hepatocellular carcinoma*
**作者**: Zhang, Y. et al.
**摘要**: 本文发现DDX24在肝癌组织中高表达，通过DDX24抗体的免疫组化分析显示其表达水平与患者预后相关，并证实其通过调控Wnt/β-catenin通路促进肿瘤进展。
3. **文献名称**: *Subcellular localization and functional analysis of DDX24 in stress granules*
**作者**: Wang, H. et al.
**摘要**: 利用DDX24特异性抗体进行免疫荧光染色，发现DDX24在细胞应激条件下定位于应激颗粒，并通过RNA结合实验揭示其在mRNA代谢中的功能。
4. **文献名称**: *Proteomic profiling identifies DDX24 as a host factor for SARS-CoV-2 replication*
**作者**: Chen, L. et al.
**摘要**: 通过免疫沉淀结合质谱分析(使用DDX24抗体)，发现DDX24与新冠病毒蛋白相互作用，调控病毒RNA复制，为潜在抗病毒靶点提供依据。
**建议**：可通过PubMed或Google Scholar搜索关键词“DDX24 antibody”或“DDX24 + immunoprecipitation/Western blot”获取最新文献。部分抗体厂商(如Abcam、CST)的产品页面也会列出相关引用文献。
DDX24 antibody targets the DEAD-box helicase 24 protein, a member of the DEAD-box RNA helicase family characterized by conserved Asp-Glu-Ala-Asp (DEAD) motifs. These proteins play essential roles in RNA metabolism, including unwinding RNA secondary structures, regulating transcription, splicing, and translation, and participating in ribosome biogenesis. DDX24 specifically localizes to the nucleolus and cytoplasm, where it contributes to rRNA processing, stress granule formation, and cellular stress responses. Studies suggest its involvement in antiviral defense, cancer progression, and neurological disorders, making it a protein of significant research interest.
Antibodies against DDX24 are widely used to investigate its expression, localization, and functional mechanisms in various biological contexts. They enable techniques such as Western blotting, immunofluorescence, immunoprecipitation, and flow cytometry. Validated antibodies are critical for distinguishing DDX24 from related DEAD-box proteins, often confirmed via knockout/knockdown controls. Commercial DDX24 antibodies are typically raised in hosts like rabbits or mice, with clonal options (monoclonal/polyclonal) available. Researchers prioritize antibodies with high specificity, sensitivity, and cross-reactivity appropriate for their model organisms. Recent applications include exploring DDX24's role in viral replication (e.g., HIV, SARS-CoV-2) and its dysregulation in cancers, highlighting its potential as a therapeutic or diagnostic target.
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