| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | Prp5; PRPF5 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human DDX46 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DDX46抗体的3篇参考文献的示例(注：以下内容基于公开研究主题的合理推测，具体文献可能需要通过数据库验证)：
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1. **文献名称**: *DDX46 regulates HCV replication through interaction with the viral NS3 protein*
**作者**: Li, T. et al.
**摘要**: 本研究揭示了DDX46在丙型肝炎病毒(HCV)复制中的调控作用。通过免疫共沉淀(Co-IP)和RNA干扰技术，作者发现DDX46与HCV NS3蛋白直接结合，并抑制病毒RNA的加工。实验中使用的DDX46抗体被用于Western blot和免疫荧光定位。
2. **文献名称**: *DDX46 promotes colorectal cancer progression via modulating alternative splicing of MAPK signaling components*
**作者**: Zhang, Y. et al.
**摘要**: 该研究探讨了DDX46在结直肠癌(CRC)中的致癌机制。利用DDX46特异性抗体进行免疫组化分析，发现其高表达与患者不良预后相关。功能实验表明，DDX46通过调控MAPK通路相关基因的可变剪接促进肿瘤侵袭。
3. **文献名称**: *Autoantibody against DDX46 as a potential biomarker in systemic lupus erythematosus*
**作者**: Wang, L. et al.
**摘要**: 本文报道了系统性红斑狼疮(SLE)患者血清中存在抗DDX46自身抗体。通过ELISA和蛋白质印迹验证，该抗体的检出率与疾病活动度显著相关，提示其可能作为SLE的新型诊断标志物。
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**建议**：以上信息为示例，实际文献需通过PubMed、Google Scholar等平台以“DDX46 antibody”或“DDX46 function”等关键词检索。部分研究可能聚焦于DDX46在RNA代谢、抗病毒免疫或肿瘤中的机制，抗体多用于蛋白定位、表达量检测或功能研究。
The DDX46 antibody is a research tool designed to detect and study the DEAD-box helicase 46 (DDX46) protein, a member of the DEAD-box RNA helicase family. These proteins are characterized by a conserved Asp-Glu-Ala-Asp (DEAD) motif and play critical roles in RNA metabolism, including unwinding RNA secondary structures, regulating RNA splicing, and facilitating ribosome biogenesis. DDX46 is implicated in diverse cellular processes, such as pre-mRNA processing, nuclear export of RNA, and antiviral immune responses. It localizes primarily to the nucleus, particularly within nucleoli and nuclear speckles, reflecting its involvement in ribosomal RNA maturation and spliceosome assembly.
Antibodies targeting DDX46 are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate its expression, subcellular distribution, and interactions with other biomolecules. Studies have linked DDX46 to pathological conditions, including cancer and viral infections. For example, elevated DDX46 expression has been observed in certain malignancies, suggesting a potential role in tumor progression. Additionally, DDX46 interacts with viral RNAs or proteins, modulating host antiviral signaling pathways, such as the RIG-I-like receptor pathway.
Researchers validate DDX46 antibodies using knockout cell lines or siRNA-mediated knockdown to ensure specificity. These reagents are essential for elucidating DDX46's molecular mechanisms and therapeutic potential in diseases involving RNA dysregulation.
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