| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POLB |
| Uniprot No | P06746 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-335aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMSKRKAPQETLNGGITDMLTELANFEKNVS QAIHKYNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIAEKIDEFLATG KLRKLEKIRQDDTSSSINFLTRVSGIGPSAARKFVDEGIKTLEDLRKNED KLNHHQRIGLKYFGDFEKRIPREEMLQMQDIVLNEVKKVDSEYIATVCGS FRRGAESSGDMDVLLTHPSFTSESTKQPKLLHQVVEQLQKVHFITDTLSK GETKFMGVCQLPSKNDEKEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNK NMRAHALEKGFTINEYTIRPLGVTGVAGEPLPVDSEKDIFDYIQWKYREP KDRSE |
| 预测分子量 | 40 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于POLB(DNA聚合酶β)重组蛋白研究的参考文献概览：
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1. **文献名称**：*Expression and characterization of human DNA polymerase β in Escherichia coli*
**作者**：Kumar, A., et al.
**摘要**：该研究报道了人源POLB基因在大肠杆菌中的重组表达及纯化策略，通过优化诱导条件获得高活性重组蛋白，并验证其在碱基切除修复中的催化功能。
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2. **文献名称**：*Structural and functional analysis of recombinant DNA polymerase β mutants*
**作者**：Date, T., et al.
**摘要**：利用定点突变技术构建多个POLB突变体，通过X射线晶体学解析其三维结构，揭示关键氨基酸残基在底物识别和催化活性中的作用。
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3. **文献名称**：*Role of DNA polymerase β in oxidative DNA damage repair*
**作者**：Wilson, S.H., et al.
**摘要**：研究重组POLB蛋白在氧化损伤修复中的功能机制，证明其通过短片段合成修复8-oxoG等氧化损伤位点，并探讨其与癌症易感性的关联。
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**备注**：以上文献为示例性概括，实际引用需根据具体研究领域补充完整信息(期刊、年份、卷号等)。建议通过PubMed或Web of Science检索关键词“recombinant DNA polymerase beta”获取最新文献。
**Background of POLB (DNA Polymerase Beta) Recombinant Protein**
DNA polymerase beta (POLB) is a key enzyme in the base excision repair (BER) pathway, a critical mechanism for maintaining genomic stability by repairing small DNA lesions caused by oxidation, alkylation, or hydrolysis. As the smallest eukaryotic DNA polymerase, POLB (~39 kDa) is primarily expressed in vertebrates and plays a specialized role in gap-filling synthesis during BER. It consists of two functional domains: an 8 kDa lyase domain that removes 5'-deoxyribose phosphate (dRP) groups and a 31 kDa polymerase domain responsible for DNA synthesis.
Recombinant POLB protein is produced via heterologous expression systems, such as *E. coli*, enabling large-scale purification for biochemical and structural studies. Its recombinant form retains enzymatic activity, making it essential for *in vitro* studies on DNA repair mechanisms, mutagenesis, and enzyme kinetics. Researchers utilize POLB to investigate its fidelity, processivity, and interactions with other BER proteins (e.g., XRCC1. AP endonuclease).
POLB dysfunction is linked to genomic instability, cancer, and neurodegenerative diseases. Mutations in POLB are associated with chemotherapy resistance in tumors, as impaired BER allows cancer cells to evade DNA-damaging treatments. Conversely, overexpression of POLB may contribute to genetic disorders due to error-prone repair.
In drug discovery, recombinant POLB serves as a target for inhibitors aimed at sensitizing cancer cells to radiotherapy or chemotherapy. Structural studies of recombinant POLB (e.g., X-ray crystallography) provide insights into substrate binding and catalysis, guiding the design of small-molecule modulators.
Overall, POLB recombinant protein is a vital tool for advancing our understanding of DNA repair biology and developing therapeutic strategies against cancer and aging-related diseases.
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