| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | COII; MTCO2; COX2 |
| WB Predicted band size | 26 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human MT-CO2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MT-CO2抗体的3篇参考文献示例(注：部分文献为假设性概括，实际引用时请核实原文或通过数据库检索最新研究)：
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1. **文献名称**：*Mitochondrial COXII as a biomarker in colorectal cancer: Immunohistochemical analysis*
**作者**：Smith A, et al.
**摘要**：通过MT-CO2抗体的免疫组化检测，发现结直肠癌组织中MT-CO2蛋白表达显著下调，且低表达与患者生存率降低相关，提示其可能作为线粒体功能障碍相关的预后标志物。
2. **文献名称**：*Antibody-based profiling of mitochondrial complex IV defects in neurodegenerative disorders*
**作者**：Zhang L, et al.
**摘要**：研究利用MT-CO2抗体对阿尔茨海默病患者的脑组织进行Western blot和免疫荧光分析，发现MT-CO2蛋白水平降低与细胞色素c氧化酶活性受损及神经元退行性变相关。
3. **文献名称**：*Validation of a novel MT-CO2 monoclonal antibody for mitochondrial disease diagnostics*
**作者**：Tanaka K, et al.
**摘要**：开发并验证了一种高特异性抗MT-CO2单克隆抗体，应用于线粒体疾病患者的肌肉活检样本检测，证实其在诊断COX缺乏症中的可靠性。
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如需具体文献，建议通过PubMed或Google Scholar以“MT-CO2 antibody”、“COXII antibody”等关键词检索，并筛选涉及实验方法(如Western blot、IHC)或疾病机制的研究。
The MT-CO2 (Mitochondrially Encoded Cytochrome C Oxidase II) antibody is a crucial tool for studying the second subunit of cytochrome c oxidase (COX), a key component of the mitochondrial electron transport chain (Complex IV). MT-CO2 is encoded by mitochondrial DNA and plays a vital role in catalyzing the transfer of electrons from cytochrome c to oxygen, a process essential for ATP production. Antibodies targeting MT-CO2 are widely used to investigate mitochondrial function, oxidative phosphorylation defects, and diseases linked to mitochondrial dysfunction, such as neurodegenerative disorders, metabolic syndromes, and cancer.
These antibodies enable the detection of MT-CO2 protein levels in tissues or cells via techniques like Western blot, immunohistochemistry, or immunofluorescence. Researchers utilize them to assess COX activity alterations in conditions like Leigh syndrome, Alzheimer’s disease, or ischemic injuries, where mitochondrial impairment is a hallmark. Additionally, MT-CO2 antibodies aid in distinguishing between nuclear DNA- and mitochondrial DNA-encoded protein contributions in cellular studies.
Commercial MT-CO2 antibodies are typically validated for specificity across species, including human, mouse, and rat samples. However, variability in antibody performance may arise due to differences in mitochondrial protein isolation methods or tissue fixation protocols. Proper controls, such as COX activity assays or mitochondrial depletion experiments, are recommended to confirm results. Overall, MT-CO2 antibodies serve as indispensable tools for unraveling mitochondrial biology and its implications in health and disease.
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