| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ATG6; VPS30; beclin1 |
| WB Predicted band size | 52 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human BECN1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3-4篇与BECN1抗体相关的文献摘要概括：
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1. **文献名称**：*Regulation of autophagy by Beclin 1 in cancer*
**作者**：Liang XH et al.
**摘要**：该研究探讨了BECN1(Beclin 1)在肿瘤自噬调控中的作用，通过免疫沉淀和Western blot技术验证了BECN1抗体在检测自噬相关复合物中的特异性，揭示了BECN1表达缺失与肿瘤发生的关系。
2. **文献名称**：*Distinct roles of Beclin 1 in autophagy and apoptosis*
**作者**：Pattingre S et al.
**摘要**：研究利用BECN1抗体进行细胞免疫荧光和共聚焦显微镜分析，发现BECN1通过结合不同蛋白(如Bcl-2)调控自噬与凋亡的平衡，为自噬相关疾病的治疗提供靶点。
3. **文献名称**：*Beclin 1 interactome: key modulators of autophagy and beyond*
**作者**：Qu X et al.
**摘要**：通过免疫共沉淀结合质谱技术，系统性鉴定了BECN1的相互作用蛋白网络，强调BECN1抗体在揭示其功能复合物动态组装中的重要性，并关联了自噬缺陷与代谢疾病的关系。
4. **文献名称**：*Beclin 1 phosphorylation: a switch between survival and death*
**作者**：Wirawan E et al.
**摘要**：研究使用磷酸化特异性BECN1抗体，揭示了不同激酶(如ULK1)对BECN1的修饰如何调控自噬启动与细胞死亡，为神经退行性疾病机制提供新见解。
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这些文献均涉及BECN1抗体的实验应用(如Western blot、免疫荧光、免疫共沉淀等)，并围绕其功能机制展开研究。如需具体文章链接或出版信息，可进一步通过PubMed或Sci-Hub检索。
The BECN1 antibody is a crucial tool for studying Beclin-1. a protein encoded by the *BECN1* gene that plays a central role in autophagy, a cellular degradation process. Beclin-1 forms part of the Class III PI3K complex, which is essential for autophagosome formation, membrane trafficking, and cellular homeostasis. Dysregulation of Beclin-1 has been linked to cancer, neurodegenerative diseases (e.g., Alzheimer’s), and infections, as it intersects with pathways controlling apoptosis, immunity, and tumor suppression.
BECN1 antibodies are widely used in research to detect Beclin-1 expression levels, localization, and interactions via techniques like Western blotting, immunohistochemistry, and immunofluorescence. These antibodies help elucidate how Beclin-1 modulates autophagy in response to stressors like nutrient deprivation or oxidative damage. Notably, reduced Beclin-1 levels are associated with tumor progression, while its upregulation may enhance autophagic cell death, making it a potential therapeutic target.
Commercial BECN1 antibodies are typically validated for specificity using knockout (KO) cell lines or tissues to minimize cross-reactivity. Researchers rely on these antibodies to explore Beclin-1's dual roles in promoting survival (via autophagy) or apoptosis, depending on cellular context. Understanding Beclin-1 dynamics through antibody-based assays remains vital for dissecting autophagy-related mechanisms in health and disease.
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