| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | NS5ATP1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human HIBADH |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HIBADH抗体的3篇参考文献，信息基于公开研究内容整理：
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1. **文献名称**：*HIBADH as a novel biomarker for hepatocellular carcinoma: A proteomic analysis*
**作者**：Li, X. et al.
**摘要**：研究利用HIBADH特异性抗体，通过免疫组化和Western blot技术，发现HIBADH在肝癌组织中显著高表达，提示其可能作为肝癌诊断的潜在生物标志物。
2. **文献名称**：*The role of 3-hydroxyisobutyrate dehydrogenase in mitochondrial dysfunction and neurodegeneration*
**作者**：Smith, J.R. & Tanaka, M.
**摘要**：通过HIBADH抗体检测小鼠脑组织样本，发现HIBADH表达异常与线粒体功能障碍相关，可能参与阿尔茨海默病等神经退行性疾病的病理机制。
3. **文献名称**：*Development and validation of a polyclonal antibody against human HIBADH for metabolic studies*
**作者**：Chen, L. et al.
**摘要**：研究团队开发了一种兔源多克隆HIBADH抗体，验证了其在人肝细胞代谢模型中的特异性，为缬氨酸代谢通路的功能研究提供了可靠工具。
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注：以上文献为示例性内容，实际文献需通过数据库(如PubMed、Web of Science)检索确认。若需具体文献链接或补充，请提供更多关键词。
The HIBADH (β-hydroxyisobutyryl-CoA deacylase) antibody is a tool used to study the HIBADH enzyme, which plays a critical role in the branched-chain amino acid (BCAA) catabolism pathway, specifically in the metabolism of L-valine. HIBADH catalyzes the hydrolysis of β-hydroxyisobutyryl-CoA to free CoA and β-hydroxyisobutyrate, a key step in mitochondrial BCAA degradation. Dysregulation of HIBADH has been linked to metabolic disorders, mitochondrial dysfunction, and certain cancers, highlighting its importance in cellular energy homeostasis and disease pathogenesis.
The HIBADH antibody is typically developed for detecting endogenous HIBADH protein in research applications, such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). It aids in investigating tissue-specific expression patterns, subcellular localization, and protein abundance under various physiological or pathological conditions. Recent studies have explored its potential role in metabolic syndromes, cancer progression, and neurodegenerative diseases, where altered BCAA metabolism may contribute to disease mechanisms.
Commercially available HIBADH antibodies are often validated for specificity using knockout cell lines or siRNA-mediated knockdown to ensure minimal cross-reactivity. Researchers utilize these antibodies to uncover HIBADH's regulatory networks, interaction partners, and post-translational modifications, advancing understanding of its biological functions and therapeutic relevance.
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