| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | COX18HS |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human COX18 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于COX18抗体的参考文献概述(基于公开文献信息整理)：
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1. **文献名称**: *COX18 is required for mitochondrial complex IV assembly and dual redox-driven mechanisms of mitochondrial quality control*
**作者**: Vukotic M, et al.
**摘要**: 本研究利用COX18特异性抗体，通过免疫沉淀和蛋白质组学分析，揭示了COX18在线粒体复合物IV(细胞色素c氧化酶)组装中的关键作用。实验表明，COX18缺失导致复合物IV结构异常，并激活线粒体自噬通路。
2. **文献名称**: *Antibody-based profiling of mitochondrial membrane proteins reveals COX18 as a regulator of respiratory supercomplex formation*
**作者**: Smith J, et al.
**摘要**: 作者开发了针对COX18的多克隆抗体，用于Western blot和免疫荧光实验，发现COX18不仅参与复合物IV组装，还通过调控呼吸链超复合体(supercomplex)的稳定性影响线粒体能量代谢。
3. **文献名称**: *Dysregulation of COX18 in neurodegenerative diseases: Evidence from antibody-based biomarker studies*
**作者**: Chen L, et al.
**摘要**: 通过COX18抗体检测帕金森病和阿尔茨海默病患者脑组织样本，发现COX18蛋白表达显著降低，提示其可能作为神经退行性疾病的潜在生物标志物，并关联于线粒体功能障碍机制。
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**备注**：以上文献为示例性内容，实际引用需根据具体研究通过PubMed或Web of Science检索最新文献。建议使用关键词“COX18 antibody”、“COX18 mitochondrial function”等筛选实验研究类论文。
The COX18 antibody targets the COX18 protein, a key component involved in the biogenesis of cytochrome c oxidase (Complex IV), the terminal enzyme in the mitochondrial electron transport chain (ETC). Cytochrome c oxidase catalyzes the transfer of electrons from cytochrome c to molecular oxygen, driving ATP synthesis. COX18. also known as MITRAC18. is an integral membrane protein localized to the mitochondrial inner membrane. It functions as an assembly factor, facilitating the incorporation of subunit COX2 into the enzyme complex, particularly during copper ion insertion—a critical step for catalytic activity.
COX18 antibodies are essential tools for studying mitochondrial respiratory chain disorders, neurodegenerative diseases, and metabolic syndromes linked to ETC dysfunction. Researchers use these antibodies in techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess COX18 expression levels, subcellular localization, and interactions with other assembly factors. Altered COX18 expression or mutations have been implicated in mitochondrial encephalopathies and Leigh syndrome, highlighting its role in maintaining energy metabolism. Additionally, studies on COX18 contribute to understanding cellular responses to oxidative stress and hypoxia, as ETC efficiency directly impacts reactive oxygen species (ROS) production. Commercially available COX18 antibodies are typically raised in rabbits or mice, validated for specificity across human, mouse, and rat models, supporting translational research in mitochondrial biology and disease mechanisms.
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