| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | CX58, CX59, GJA10 |
| WB Predicted band size | 59 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human GJA9 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GJA9(Connexin 59.缝隙连接蛋白)抗体的3篇代表性文献示例(注：因GJA9研究相对较少，部分文献信息可能为示例性概括)：
1. **《Connexin 59 expression and antibody characterization in mouse testis》**
- **作者**: Smith A, et al.
- **摘要**: 研究利用新开发的兔源多克隆抗体，首次在小鼠睾丸组织中检测到GJA9蛋白的表达，发现其在精子发生过程中的动态定位变化，提示其可能在生殖细胞间通讯中发挥作用。
2. **《Role of GJA9 in osteoblast differentiation: Insights from antibody-mediated inhibition experiments》**
- **作者**: Zhang Y, et al.
- **摘要**: 通过抗GJA9的单克隆抗体阻断功能实验，发现GJA9蛋白在成骨细胞分化中调控钙信号传递，为骨代谢疾病研究提供了潜在靶点。
3. **《Development of a specific ELISA assay for GJA9 using novel monoclonal antibodies》**
- **作者**: Tanaka K, et al.
- **摘要**: 报道了一种高特异性小鼠抗人GJA9单克隆抗体的开发，并基于此建立ELISA检测方法，应用于癌症患者血清中GJA9的定量分析，发现其与某些肿瘤转移相关。
(注：因GJA9研究有限，以上文献为假设性示例。实际研究中GJA9可能被更广泛称为**Cx59**或存在物种特异性命名差异，建议通过PubMed等数据库以“GJA9 antibody”或“Connexin 59”为关键词检索最新文献。)
The GJA9 antibody targets the gap junction protein alpha 9 (GJA9), also known as connexin 59 (Cx59) in some species or connexin 50 (Cx50) in humans, a member of the connexin family. Connexins form intercellular channels called gap junctions, enabling direct communication between adjacent cells by transferring ions, metabolites, and signaling molecules. GJA9 is primarily expressed in the lens of the eye, where it plays a critical role in maintaining lens transparency and homeostasis. Mutations in the GJA9 gene have been linked to congenital cataracts, highlighting its importance in ocular physiology.
GJA9 antibodies are essential tools in studying the protein’s expression, localization, and function. They are widely used in techniques like immunohistochemistry, Western blotting, and immunofluorescence to investigate GJA9 distribution in tissues, particularly in lens epithelial cells and fiber cells. Research utilizing these antibodies has advanced understanding of gap junction dynamics in lens development, cataractogenesis, and potential regenerative therapies.
Beyond the eye, GJA9 antibodies have exploratory applications in detecting ectopic expression in other tissues, though its primary role remains ocular-focused. Studies also employ these antibodies to probe pathological mechanisms in genetically engineered animal models of cataracts. Despite its niche focus, GJA9 research underscores the broader significance of connexins in cellular communication and disease, bridging basic science and clinical insights into vision disorders.
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