| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/10000 | Human,Mouse,Rat |
| Aliases | AQP-8 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human AQP8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于AQP8抗体的3篇参考文献示例(注：以下为虚构内容，仅用于示例格式)：
1. **文献名称**: "Immunolocalization and Functional Analysis of Aquaporin-8 in the Mouse Intestinal Epithelium"
**作者**: Garcia, F. et al.
**摘要**: 本研究通过特异性AQP8抗体，利用免疫组化和Western blot技术，揭示了AQP8在小鼠结肠上皮细胞顶膜的高表达，并证实其在肠道水分转运和渗透调节中的关键作用。
2. **文献名称**: "AQP8 Expression in Human Liver: Role in Bile Secretion and Cholestatic Disease"
**作者**: Chen, L. et al.
**摘要**: 通过免疫荧光和AQP8抗体标记，研究发现AQP8在人类肝细胞胆管膜上显著表达，其表达水平降低与胆汁淤积性疾病相关，提示AQP8可能参与胆汁分泌的分子调控。
3. **文献名称**: "Aquaporin-8 as a Potential Biomarker in Colorectal Cancer: Antibody-Based Detection and Clinical Correlations"
**作者**: Zhang, Y. et al.
**摘要**: 使用AQP8抗体的免疫组化分析显示，结直肠癌组织中AQP8表达显著下调，且低表达与患者预后不良相关，提示AQP8可能在肿瘤细胞代谢中起抑制作用。
4. **文献名称**: "AQP8 Antibody Reveals Mitochondrial Localization and Apoptosis Regulation in Renal Cells"
**作者**: Wang, H. et al.
**摘要**: 通过AQP8抗体的亚细胞定位研究，发现AQP8不仅存在于细胞膜，还定位于肾小管上皮细胞线粒体，其过表达可减少氧化应激诱导的细胞凋亡，提示其双重功能。
(注：实际文献需通过PubMed、Google Scholar等平台检索确认。)
Aquaporin-8 (AQP8) antibody is a research tool designed to detect and study the expression and function of aquaporin-8. a member of the aquaporin family of transmembrane water channel proteins. AQP8 facilitates the selective transport of water and small neutral solutes across cell membranes, playing roles in osmotic balance, fluid secretion, and cellular hydration. It is expressed in various tissues, including the liver, pancreas, kidneys, gastrointestinal tract, and reproductive organs, where it contributes to organ-specific physiological processes such as bile production, pancreatic secretion, and sperm motility.
AQP8 antibodies are typically developed in immunized hosts (e.g., rabbits, mice) using peptide sequences or recombinant proteins corresponding to specific epitopes of the AQP8 protein. These antibodies are validated for applications such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry to localize and quantify AQP8 expression in tissues or cultured cells. Researchers utilize AQP8 antibodies to investigate its involvement in pathological conditions, such as cholestasis, pancreatitis, cystic fibrosis, or infertility, where dysregulation of water transport may contribute to disease mechanisms. Commercial AQP8 antibodies often specify reactivity across species (e.g., human, mouse, rat) and provide validation data (e.g., knockout controls) to ensure specificity. Despite their utility, careful optimization of experimental conditions is required to minimize cross-reactivity with other aquaporin isoforms (e.g., AQP3. AQP9) due to structural similarities within the aquaporin family.
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