| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/15-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | ASD2, VSD1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human GATA4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3-4篇关于GATA4抗体的代表性文献示例(内容为模拟概括，具体引用请以实际文献为准)：
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1. **文献名称**: *GATA4 mutations in human congenital heart disease*
**作者**: Kuo CT, et al.
**摘要**: 研究利用GATA4特异性抗体分析先天性心脏病患者心脏组织中GATA4蛋白的表达和定位，发现突变导致其转录活性下降，影响心脏发育相关基因调控。
2. **文献名称**: *The transcription factor GATA4 is required for follicular development and normal ovarian function*
**作者**: Watt AJ, et al.
**摘要**: 通过GATA4抗体进行免疫组化和Western blot实验，揭示GATA4在卵巢卵泡发育中的关键作用，其缺失导致生殖细胞分化异常。
3. **文献名称**: *GATA4 cooperates with NKX2-5 to regulate cardiac-specific gene expression*
**作者**: Molkentin JD, et al.
**摘要**: 使用GATA4抗体进行ChIP-seq和共沉淀实验，证明GATA4与NKX2-5协同调控心肌细胞分化，并参与心脏发育的分子机制。
4. **文献名称**: *GATA4 as a diagnostic marker for gastrointestinal stromal tumors*
**作者**: Hirose T, et al.
**摘要**: 通过GATA4抗体的免疫染色分析，发现其在胃肠道间质瘤中的高特异性表达，提示其作为潜在肿瘤标志物的临床应用价值。
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注：以上文献信息为示例性概括，实际研究请通过PubMed或Web of Science等平台检索关键词“GATA4 antibody”获取准确文献。
The GATA4 antibody is a widely used tool in molecular and developmental biology research, targeting the GATA4 transcription factor—a critical regulator of tissue-specific gene expression. Belonging to the GATA family of zinc finger proteins, GATA4 binds to DNA motifs containing the consensus sequence "GATA," playing essential roles in embryonic development, particularly in cardiac morphogenesis, gut formation, and gonadal differentiation. It also regulates genes involved in cell proliferation, survival, and differentiation in adult tissues.
GATA4 antibodies are typically produced in hosts like rabbits or mice using immunogenic peptides derived from conserved regions of the human or mouse GATA4 protein. These antibodies enable detection and localization of GATA4 in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Validated antibodies show specificity for GATA4 over other GATA family members (e.g., GATA6) to avoid cross-reactivity.
In research, GATA4 antibodies help investigate congenital heart defects, reproductive disorders, and cancers (e.g., ovarian, testicular, and lung tumors) where GATA4 expression is dysregulated. They also aid in studying cellular reprogramming and organoid development. Proper validation using knockout controls or siRNA-mediated knockdown is critical, as off-target signals can arise due to protein homology. Commercial antibodies often provide data on species reactivity, application suitability, and recommended dilutions to ensure reproducibility.
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