| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/10000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human GATA6 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于GATA6抗体的3篇文献概览，包含文献名称、作者及摘要内容概括：
---
1. **文献名称**：*GATA6 is critical for pancreatic differentiation and function*
**作者**：Xuan, S., et al.
**摘要**：研究利用GATA6抗体通过免疫组化分析，揭示GATA6在小鼠胰腺发育中的关键作用，证明其缺失导致内分泌细胞分化异常及胰岛素分泌缺陷。
2. **文献名称**：*GATA6 expression in colorectal cancer: a novel biomarker for prognosis*
**作者**：Kim, M., et al.
**摘要**：通过Western blot和免疫组化检测结直肠癌组织中GATA6蛋白表达，发现高表达GATA6与患者生存率负相关，提示其作为预后标志物的潜力。
3. **文献名称**：*Antibody validation for chromatin immunoprecipitation of GATA6 in embryonic stem cells*
**作者**：Zhang, Y., et al.
**摘要**：评估多种GATA6抗体的特异性，筛选出适用于ChIP实验的可靠抗体，并证实GATA6在胚胎干细胞多能性维持中的调控作用。
---
以上文献涵盖发育生物学、癌症研究及抗体验证领域，为GATA6功能研究和实验方法提供参考。如需具体期刊信息或发表年份，可进一步补充检索关键词(如“GATA6 antibody validation”或“GATA6 cancer”)。
The GATA6 antibody is a crucial tool in studying the GATA-binding protein 6 (GATA6), a member of the GATA transcription factor family characterized by zinc finger DNA-binding domains. GATA6 plays a pivotal role in embryonic development, particularly in endoderm differentiation, cardiac morphogenesis, and the development of organs such as the pancreas, intestines, and lungs. It regulates gene expression by binding to the consensus DNA sequence (A/T)GATA(A/G) in promoter/enhancer regions, influencing cell differentiation, proliferation, and survival. Dysregulation of GATA6 is linked to diseases, including cancers (e.g., pancreatic, colorectal), diabetes, and congenital defects.
GATA6 antibodies are widely used in research to detect and quantify GATA6 protein expression in tissues or cells via techniques like immunohistochemistry (IHC), Western blot (WB), and immunofluorescence (IF). These antibodies are typically raised in hosts such as rabbits or mice, targeting specific epitopes of human, mouse, or rat GATA6. Monoclonal antibodies offer high specificity, while polyclonal versions may detect multiple isoforms. Validation parameters include reactivity across species, application suitability, and cross-reactivity checks. Researchers employ GATA6 antibodies to explore developmental pathways, disease mechanisms, and potential therapeutic targets, especially in contexts like tumor progression or organogenesis studies. Mutations or altered expression of GATA6 are also associated with pancreatic agenesis and congenital heart defects, underscoring its biomedical relevance.
×
