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产品描述：人封闭蛋白5(CLDN5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CLDN5含量。CLDN5 即闭合蛋白 5，是紧密连接蛋白家族成员，位于血管内皮细胞间。它对维持血脑屏障完整性至关重要。研究多围绕其在肿瘤血管生成、血脑屏障通透性调节等机制展开，旨在为肿瘤诊断、治疗及药物递送提供新方向。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品适用于研究血脑屏障功能、血管内皮细胞生物学特性、炎症微环境调控等基础科研领域，例如探索缺血性脑卒中、脑肿瘤或神经退行性疾病中CLDN5的动态变化，或评估药物干预对血管通透性的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CLDN5; AWAL; TMVCF; Claudin-5; Transmembrane protein deleted in VCFS; TMDVCF
缩写：CLDN5
Uniprot No.：O00501
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CLDN5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 88-97 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 94-107 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 87-99 | |||||
回收率：
| The recovery of human CLDN5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 102 | 95-106 | ||||
| EDTA plasma (n=4) | 95 | 89-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20001.9302.0121.9711.85010001.4961.5981.5471.4265001.0531.0681.0610.9402500.7320.7560.7440.6231250.4440.4350.4400.31962.50.3120.3260.3190.19831.250.2170.2010.2090.08800.1220.1200.121 | ||||||
货期：3-5 working days
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