首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠四连接素(CLEC3B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CLEC3B含量。CLEC3B，也称为四连蛋白，是一种分泌的同源三聚体蛋白，主要存在于血浆中。它能够与纤溶酶原和kringle 4结合，可能参与胞吐作用的分子包装。CLEC3B的表达与癌症进展和不良预后相关，可能通过调节免疫细胞浸润和抑制细胞增殖来影响肿瘤发展。试剂盒检测范围为1.56 ng/mL-100 ng/mL，该产品广泛应用于肿瘤生物学、细胞信号转导等领域的实验研究，特别适用于基因敲除/过表达模型的蛋白表达验证、疾病动物模型的分子标志物动态监测等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Clec3b ELISA Kit; Tna ELISA Kit; Tetranectin ELISA Kit; TN ELISA Kit; C-type lectin domain family 3 member B ELISA Kit; Plasminogen kringle 4-binding protein ELISA Kit
缩写：CLEC3B
Uniprot No.：P43025
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CLEC3B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 104 | |||||
| Range % | 100-112 | ||||||
| 1:200 | Average % | 90 | |||||
| Range % | 86-94 | ||||||
| 1:400 | Average % | 86 | |||||
| Range % | 82-90 | ||||||
| 1:800 | Average % | 93 | |||||
| Range % | 89-98 | ||||||
回收率：
| The recovery of mouse CLEC3B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 90-100 | |||||
| EDTA plasma (n=4) | 88 | 84-92 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 1002.2212.3802.3012.162 501.9752.0141.9951.856 251.6161.5281.5721.433 12.51.0040.9790.9920.853 6.250.6340.6520.6430.504 3.120.3920.4070.4000.261 1.560.2500.2430.2470.108 00.1420.1350.139 | |||||||
货期：3-5 working days
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