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产品描述：猪辅脂酶(CLPS)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CLPS含量。试剂盒检测范围为15.6 mU/mL-1000 mU/mL，该产品适用于动物营养代谢研究、消化系统疾病机制探索、饲料添加剂功效评估等科研场景，特别在猪模型研究中可量化评估胰腺功能或脂肪吸收状态本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CLPS ELISA Kit; Colipase ELISA Kit; Procolipase II ELISA Kit
缩写：CLPS
Uniprot No.：P02703
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 mU/mL-1000 mU/mL
灵敏度：3.9 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig CLPS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 87 | |||||
| Range % | 82-92 | ||||||
| 1:2 | Average % | 99 | |||||
| Range % | 94-103 | ||||||
| 1:4 | Average % | 103 | |||||
| Range % | 98-107 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 90-101 | ||||||
回收率：
| The recovery of pig CLPS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 85-105 | |||||
| EDTA plasma (n=4) | 104 | 88-111 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| mU/mlOD1OD2AverageCorrected10002.2972.3122.3052.1965001.7861.6881.7371.6282501.1331.2021.1681.0591250.6540.6650.6600.55162.50.3890.3740.3820.27331.20.2420.2540.2480.13915.60.1770.1870.1820.07300.1050.1130.109 | |||||||
货期：3-5 working days
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