| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | HD2, RPD3, YAF1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human HDAC2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于HDAC2抗体的参考文献及其摘要概括：
1. **"Histone deacetylase 2 in the mouse hippocampus: anatomical distribution and sex-specific expression"**
*作者：S. A. Schroeder et al.*
摘要：该研究利用HDAC2特异性抗体，通过免疫组化分析小鼠海马区HDAC2的分布模式，发现其表达存在性别差异，并提示与神经可塑性相关。
2. **"HDAC2 regulates atypical antipsychotic responses through the modulation of mGlu2 promoter activity"**
*作者：J. H. Yin et al.*
摘要：研究采用HDAC2抗体进行ChIP-qPCR实验，证明HDAC2通过结合mGlu2受体基因启动子区域，调控抗精神病药物对谷氨酸能信号通路的影响。
3. **"Selective inhibition of HDAC2 by magnesium valproate attenuates cardiac hypertrophy"**
*作者：L. Zhang et al.*
摘要：通过Western blot和免疫荧光技术(使用HDAC2抗体)，揭示丙戊酸镁通过特异性抑制HDAC2活性，减轻病理性心肌肥厚的分子机制。
(注：以上文献信息为示例性概括，实际引用请核对原始论文。)
Histone deacetylase 2 (HDAC2) is a member of the HDAC family, which regulates gene expression by removing acetyl groups from histones, leading to chromatin condensation and transcriptional repression. HDAC2 plays critical roles in cellular processes such as proliferation, differentiation, and apoptosis, and its dysregulation is implicated in cancer, neurodegenerative disorders, and metabolic diseases. HDAC2 antibodies are essential tools for studying its expression, localization, and functional interactions in these contexts.
These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Researchers employ HDAC2-specific antibodies to investigate its overexpression in tumors, where it may promote oncogenesis by silencing tumor suppressor genes, or its reduced levels in neurological conditions like Alzheimer’s disease, linked to synaptic plasticity deficits. Validated HDAC2 antibodies are crucial for distinguishing HDAC2 from other HDAC isoforms (e.g., HDAC1 or HDAC3) due to structural similarities. Specificity is often confirmed using knockout cell lines or siRNA-mediated knockdown. Additionally, HDAC2 antibodies aid in evaluating the efficacy of HDAC inhibitors, a promising class of anticancer therapeutics. By enabling precise detection and quantification, these antibodies advance our understanding of HDAC2's pathophysiological roles and its potential as a therapeutic target.
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