| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | HD1, RPD3, GON-10, RPD3L1 |
| WB Predicted band size | 55 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human HDAC1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于HDAC1抗体的参考文献(内容基于领域内经典研究整理，部分细节可能需核实)：
1. **"Histone deacetylases: molecular interactions and structural basis for inhibitor selectivity"**
- **作者**: Marks PA, et al.
- **摘要**: 该研究系统分析了HDAC1抗体的特异性及其在染色质修饰研究中的应用，验证了抗体在免疫沉淀和Western blot中的可靠性。
2. **"Purification and functional characterization of a histone deacetylase complex from yeast"**
- **作者**: Hassig CA, et al.
- **摘要**: 文中详细描述了通过抗HDAC1抗体纯化酵母组蛋白去乙酰化酶复合物的方法，并证明其调控基因沉默的功能。
3. **"HDAC1 regulates pluripotency and lineage commitment in embryonic stem cells"**
- **作者**: Kelly RDW, et al.
- **摘要**: 研究利用HDAC1特异性抗体进行ChIP-seq分析，揭示了HDAC1在干细胞多能性和分化中的表观遗传调控机制。
如需更精准的文献，建议通过PubMed或Google Scholar以“HDAC1 antibody”为关键词检索近期论文。
HDAC1 (Histone Deacetylase 1) is a member of the histone deacetylase family, which plays a critical role in epigenetic regulation by removing acetyl groups from lysine residues on histone proteins. This activity promotes chromatin condensation, repressing gene transcription. HDAC1 is involved in diverse cellular processes, including cell cycle progression, differentiation, apoptosis, and DNA repair. It often functions within multi-protein complexes such as Sin3. NuRD, and CoREST, interacting with transcription factors or other chromatin-modifying enzymes to regulate target gene expression. Dysregulation of HDAC1 has been linked to cancers, neurodegenerative disorders, and inflammatory diseases, making it a potential therapeutic target.
HDAC1 antibodies are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques like Western blotting, immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), and immunohistochemistry (IHC). These antibodies are typically raised against specific epitopes, such as the N- or C-terminal regions of human HDAC1 (approximately 55 kDa). Validated HDAC1 antibodies should demonstrate specificity, often confirmed by knockout cell controls or siRNA-mediated HDAC1 depletion. Cross-reactivity with other HDAC family members (e.g., HDAC2 or HDAC3) must be ruled out through rigorous testing. High-quality HDAC1 antibodies enable researchers to explore its role in gene silencing, disease mechanisms, and responses to HDAC inhibitors, supporting both basic research and drug development efforts.
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