| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | BCNG2, HAC-1, BCNG-2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human HCN2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HCN2抗体的3篇参考文献示例(文献信息为模拟，非真实存在，仅供格式参考)：
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1. **文献名称**：*HCN2 Channel Distribution in Cardiac Pacemaker Cells Revealed by Immunohistochemistry*
**作者**：Smith A, et al.
**摘要**：本研究利用特异性HCN2抗体标记心脏窦房结细胞，发现HCN2蛋白在起搏细胞膜上高度富集，表明其在心脏自律性调控中的关键作用。抗体特异性通过基因敲除小鼠模型验证。
2. **文献名称**：*Role of HCN2 in Neuropathic Pain: Insights from Antibody-Based Blockade Experiments*
**作者**：Chen L, et al.
**摘要**：通过HCN2抗体阻断大鼠背根神经节中的HCN2通道，实验显示神经痛行为显著减轻，提示HCN2可能成为慢性疼痛治疗的潜在靶点。抗体特异性通过Western blot和免疫荧光确认。
3. **文献名称**：*Development and Validation of a Novel HCN2-Specific Monoclonal Antibody*
**作者**：Yamamoto K, et al.
**摘要**：报道一种新型HCN2单克隆抗体的开发，经ELISA和免疫组化验证其对HCN2亚型的高选择性，且不与HCN1/HCN3交叉反应，适用于脑组织中的HCN2表达定位研究。
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如需真实文献，建议通过PubMed或Google Scholar搜索关键词“HCN2 antibody”或“HCN2 channel”，并筛选涉及抗体应用的研究。
The hyperpolarization-activated cyclic nucleotide-gated (HCN) channel family, comprising four isoforms (HCN1-4), regulates electrical activity in excitable tissues. HCN2. encoded by the *HCN2* gene, is a voltage-gated ion channel that contributes to pacemaker currents (Ih) in the heart and brain. It is activated by membrane hyperpolarization and modulated by cyclic nucleotides (e.g., cAMP), linking cellular signaling to electrical excitability. In the heart, HCN2 influences sinoatrial node activity and heart rate regulation. In neurons, it modulates resting membrane potential, synaptic integration, and rhythmic activity in thalamocortical and hippocampal circuits.
HCN2 antibodies are essential tools for studying the expression, localization, and function of HCN2 channels. These antibodies are typically developed using immunogenic peptides derived from specific regions of the HCN2 protein (e.g., N-terminal, C-terminal, or intracellular domains). Validation involves techniques like Western blotting, immunohistochemistry, and knockout tissue controls to ensure specificity. Applications include investigating HCN2 distribution in cardiac tissue, neuronal subtypes, or disease models, such as epilepsy, neuropathic pain, or cardiac arrhythmias. Dysregulation of HCN2 has been implicated in pathologies like sinus bradycardia and absence seizures, making its antibody a critical reagent for both basic research and therapeutic target validation. Commercial HCN2 antibodies vary in host species, clonality, and conjugation, enabling flexible experimental designs.
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