首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子Ⅱ型胶原(Col Ⅱ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的Col 含量。试剂盒检测范围为6.25 ng/mL-400 ng/mL，适用于科研场景中软骨相关疾病模型研究，如关节炎病理机制探索、软骨损伤修复评估或药物干预效果验证等领域，为组织工程、生物材料开发及退行性疾病研究提供可靠的检测工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Collagen Type 2,Col 2,_x000D_ Collagen Type II,Col II
缩写：Col
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma
检测范围：6.25 ng/mL-400 ng/mL
灵敏度：1.56 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Col Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 90-102 | |||||
| 1:2 | Average % | 107 | ||||
| Range % | 97-111 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 82-92 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 91-102 | |||||
回收率：
| The recovery of rabbit Col Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 82-94 | ||||
| EDTA plasma (n=4) | 102 | 92-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 4002.5762.5282.5522.457 2001.9491.9861.9681.873 1001.4211.3661.3941.299 500.8270.8110.8190.724 250.4340.4170.4260.331 12.50.3140.3020.3080.213 6.250.2340.2210.2280.133 00.0930.0970.095 | ||||||
货期：3-5 working days
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