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产品描述：人I型胶原α-2链(COL1A2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的COL1A2含量。COL1A2编码胶原蛋白α2链，是细胞外基质主要成分。其表达异常与多种疾病如纤维化、肿瘤相关。研究机制上，调控COL1A2表达可影响细胞增殖、迁移等。深入了解它在疾病发生发展过程的作用机制，有望为相关疾病治疗提供新靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于实验室对纤维化模型（如肝纤维化、肺纤维化）的分子机制研究、药物干预效果评估，或通过组织匀浆分析胶原代谢异常相关疾病。适用于生命科学领域对胶原蛋白动态变化的定量分析需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Alpha 2 collagen type I ELISA Kit; Alpha 2 type I collagen ELISA Kit; Alpha 2 type I procollagen ELISA Kit; Alpha 2(I) collagen ELISA Kit; Alpha 2(I) procollagen ELISA Kit; Alpha-2 type I collagen ELISA Kit; CO1A2_HUMAN ELISA Kit; COL1A2 ELISA Kit; Collagen alpha 2(I) chain ELISA Kit; Collagen alpha-2(I) chain ELISA Kit; Collagen I alpha 2 polypeptide ELISA Kit; Collagen of skin tendon and bone alpha 2 chain ELISA Kit; Collagen type I alpha 2 ELISA Kit; OI4 ELISA Kit; Osteogenesis imperfecta type IV ELISA Kit; Type I procollagen ELISA Kit
缩写：COL1A2
Uniprot No.：P08123
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human COL1A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-93 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 98-106 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of human COL1A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 85-96 | ||||
| EDTA plasma (n=4) | 104 | 98-110 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.5492.6352.5922.475102.1132.2442.1792.06251.4271.4861.4571.3402.50.8990.9310.9150.7981.250.5330.5110.5220.4050.6250.3640.3420.3530.2360.3120.2270.2180.2230.10600.1130.1210.117 | ||||||
货期：3-5 working days
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