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Mouse C-Peptide ELISA Kit

  • 中文名： 小鼠C肽(C-Peptide)ELISA Kit
货号: IPD-E05068m
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    小鼠C肽(C-Peptide)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的C-Peptide含量。C-Peptide即C肽，是胰岛素原裂解产生的与胰岛素等分子的肽类物质。研究发现它有独立于胰岛素的生物学作用，可改善神经、肾脏等器官的血流，调节细胞内信号传导，其机制为结合特定细胞膜受体，激活下游信号通路，正被深入研究以挖掘更多临床价值。试剂盒检测范围为2.86 ng/mL-50 ng/mL，糖尿病模型研究、胰岛素分泌机制探索及代谢调控实验、胰岛素相关代谢研究、糖尿病动物模型评估以及药物干预效果分析、科研领域对β细胞功能动态监测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 缩写：
    C-Peptide
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma
  • 检测范围：
    3.125 ng/ml-50 ng/ml
  • 灵敏度：
    0.625 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%


    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%


    Three samples of known concentration were tested in twenty assays to assess.








  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse C-Peptide in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    		SampleSerum(n=4)
    1:1Average %93
    Range %85-99
    1:2Average %90
    Range %85-96
    1:4Average %95
    Range %87-106
    1:8Average %95
    Range %89-100
  • 回收率：
    The recovery of mouse C-Peptide spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5)9689-100
    EDTA plasma (n=4)9591-103














  • 本试剂盒所含材料：



    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-mouse C-peptide antibody.

    • Six vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial HRP-conjugated C-peptide antibody(6 ml/bottle) ---Bind to the C-peptide in the samples or standards and react with the substrate to make the solution chromogenic.

    • One vial Wash Buffer (20x concentrate) (15ml/bottle) ---Wash away unbound or free substances.

    • Substrate A (1 x 7 ml)

    • Substrate B (1 x 7 ml)

    • 本试剂盒不含材料：



      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm or 630 nm.

      • An incubator can provide stable incubation conditions up to 37°C±5°C.

      • Centrifuge

      • Vortex

      • Squirt bottle, manifold dispenser, or automated microplate washer

      • Absorbent paper for blotting the microtiter plate


      • 货期：
        3-5 working days

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