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产品描述：猪C肽(C-Peptide)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C-Peptide含量。C-Peptide即C肽，是胰岛素原裂解产生的连接肽。其背景在于可反映胰岛β细胞功能，不受外源性胰岛素影响。研究机制方面，主要围绕评估胰岛素分泌、辅助糖尿病分型等，还在探索其对改善神经、血管病变等的潜在作用。试剂盒检测范围为1.25 ng/mL-80 ng/mL，适用于实验室条件下动态监测猪胰岛功能状态、评估糖尿病动物模型干预效果或探究代谢性疾病相关分子机制。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：C-Peptide
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：1.25 ng/mL-80 ng/mL
灵敏度：0.312 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig C-Peptide in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 88-98 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-102 | |||||
| 1:4 | Average % | 103 | ||||
| Range % | 97-107 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 81-93 | |||||
回收率：
| The recovery of pig C-Peptide spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 93-104 | ||||
| EDTA plasma (n=4) | 92 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected802.6632.5622.6132.487402.0932.2142.1542.028201.5101.5311.5211.395101.0431.0741.0590.93350.6340.6230.6290.5032.50.3810.3700.3760.2501.250.2480.2440.2460.12000.1270.1250.126 | ||||||
货期：3-5 working days
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