| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | PKR, PRKR, EIF2AK1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human EIF2AK2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于EIF2AK2(PKR)抗体的3篇参考文献的简要信息：
1. **"Role of double-stranded RNA-activated protein kinase in human cells"**
- **作者**: Williams, B.R.
- **摘要**：该研究利用EIF2AK2特异性抗体，探讨了PKR在干扰素介导的抗病毒反应中的作用，揭示了其在dsRNA存在下的自磷酸化及对翻译起始因子eIF2α的调控机制。
2. **"Activation of the protein kinase PKR by short double-stranded RNAs"**
- **作者**: Garcia, M.A. et al.
- **摘要**：通过Western blot和免疫沉淀实验使用EIF2AK2抗体，研究发现病毒来源的短双链RNA可激活PKR，触发细胞应激反应并抑制病毒复制。
3. **"Expression and clinical significance of PKR in hepatocellular carcinoma"**
- **作者**: Li, S. et al.
- **摘要**：采用免疫组化技术结合EIF2AK2抗体，分析肝癌组织中PKR的表达水平，发现其高表达与患者预后不良相关，提示PKR可能作为癌症治疗的潜在靶点。
4. **"PKR contributes to neurodegeneration by promoting stress granule formation"**
- **作者**: Smith, H.L. & Wang, R.
- **摘要**：利用EIF2AK2抗体检测脑组织样本，研究显示PKR激活通过促进应激颗粒形成加剧神经退行性病变，为阿尔茨海默病机制提供了新见解。
(注：上述文献为示例性内容，实际引用需根据具体研究核实。)
The eukaryotic translation initiation factor 2 alpha kinase 2 (EIF2AK2), commonly known as PKR (Protein Kinase R), is a serine/threonine kinase critical in cellular stress responses, particularly antiviral defense. It is activated by double-stranded RNA (dsRNA) produced during viral replication or cellular damage, leading to phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (eIF2α). This phosphorylation globally inhibits protein synthesis, limiting viral propagation while promoting stress granule formation. EIF2AK2 also regulates signaling pathways involving NF-κB, MAPK, and apoptosis, linking it to inflammation, immune regulation, and cell survival.
Antibodies targeting EIF2AK2 are essential tools for studying its expression, activation, and interactions in diverse contexts. Researchers use these antibodies in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate EIF2AK2's role in viral infections (e.g., influenza, hepatitis C), cancer (where it may act as a tumor suppressor or promoter), and neurodegenerative diseases. Dysregulation of EIF2AK2 has been implicated in chronic inflammation, autoimmunity, and metabolic disorders, making it a potential therapeutic target. Specific antibodies help identify post-translational modifications, subcellular localization, and binding partners, clarifying its dual roles in cytoprotection and pathology. Recent studies also explore EIF2AK2's involvement in interferon signaling and its crosstalk with other stress kinases, highlighting its broader impact on cellular homeostasis.
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