首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠结缔组织生长因子(CTGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的CTGF含量。CTGF（结缔组织生长因子）是一种由血管内皮细胞产生的蛋白质，主要功能包括促进细胞增殖、分化，刺激软骨细胞增殖和分化，以及诱导细胞凋亡等。在正常生理过程中，如胚胎发生、组织修复等发挥重要作用。近年来，研究发现CTGF在多种疾病的发生发展中扮演关键角色，如糖尿病视网膜病变、肝纤维化、肿瘤等。试剂盒检测范围为0.78 pg/mL-50 pg/mL，适用于纤维化疾病机制研究、心血管或肾脏疾病动物模型评估、细胞因子网络调控实验等科研场景，特别适合在体外培养体系或体内模型中追踪CTGF的动态表达变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ccn2 ELISA Kit; betaIG-M2 ELISA Kit; Ctgf ELISA Kit; Fisp-12 ELISA Kit; Fisp12 ELISA Kit; Hcs24 ELISA Kit; CCN family member 2 ELISA Kit; Cellular communication network factor 2 ELISA Kit; Connective tissue growth factor ELISA Kit; Hypertrophic chondrocyte-specific protein 24 ELISA Kit; Protein FISP-12 ELISA Kit
缩写：CTGF
Uniprot No.：P29268
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.78 pg/mL-50 pg/mL
灵敏度：0.195 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CTGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:100 | Average % | 92 | |
| Range % | 87-96 | ||
| 1:200 | Average % | 95 | |
| Range % | 90-100 | ||
| 1:400 | Average % | 87 | |
| Range % | 82-93 | ||
| 1:800 | Average % | 90 | |
| Range % | 84-96 |
回收率：
| The recovery of mouse CTGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-102 | ||||
| EDTA plasma (n=4) | 94 | 89-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 502.5972.5802.5892.412 252.2632.2982.2812.104 12.51.7791.7991.7891.612 6.251.2171.2521.2351.058 3.120.6900.6740.6820.505 1.560.4580.4270.4430.266 0.780.2850.2970.2910.114 00.1760.1780.177 | ||||||
货期：3-5 working days
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