首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠细胞毒性T淋巴细胞相关抗原4(CTLA-4/CD152)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CTLA4含量。CTLA4（细胞毒性T淋巴细胞抗原4）是一种免疫检查点蛋白，主要表达在T细胞表面，通过与其配体CD80/CD86结合，抑制T细胞活性，从而调节免疫反应。研究显示，CTLA4在肿瘤免疫逃逸中起关键作用，靶向CTLA4的免疫检查点抑制剂可增强抗肿瘤免疫反应。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于免疫机制研究、药物开发中的靶向分子评估以及动物模型中的免疫状态分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ctla4 ELISA Kit; Cd152 ELISA Kit; Cytotoxic T-lymphocyte protein 4 ELISA Kit; Cytotoxic T-lymphocyte-associated antigen 4 ELISA Kit; CTLA-4 ELISA Kit; CD antigen CD152 ELISA Kit
缩写：CTLA4
Uniprot No.：P09793
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CTLA-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 97 | |||||
| Range % | 95-99 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 97-106 | ||||||
| 1:4 | Average % | 86 | |||||
| Range % | 83-89 | ||||||
| 1:8 | Average % | 94 | |||||
| Range % | 90-98 | ||||||
回收率：
| The recovery of mouse CTLA-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 102 | 96-108 | |||||
| EDTA plasma (n=4) | 87 | 83-90 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected202.9342.8682.9012.725102.3122.3012.3072.13151.5371.6021.5701.3942.50.9510.9870.9690.7931.250.6370.6290.6330.4570.6250.3860.3740.3800.2040.3120.2980.3000.2990.12300.1750.1770.176 | |||||||
货期：3-5 working days
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