首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠组织蛋白酶B(CTSB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CTSB含量。CTSB即组织蛋白酶B，是溶酶体半胱氨酸蛋白酶家族成员。它参与多种生理过程如蛋白质代谢等。在病理方面，与肿瘤侵袭转移、神经退行性疾病等相关。研究机制主要聚焦于其活性调节、底物识别，及与疾病信号通路的相互作用。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于探索CTSB在细胞代谢调控、免疫微环境分析、疾病动物模型构建等基础研究中的应用，例如评估药物干预下组织蛋白酶活性变化、肿瘤微环境中蛋白酶动态表达或神经退行性疾病相关机制研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ctsb ELISA Kit; Cathepsin B ELISA Kit; EC 3.4.22.1 ELISA Kit; Cathepsin B1) [Cleaved into: Cathepsin B light chain; Cathepsin B heavy chain] ELISA Kit
缩写：CTSB
Uniprot No.：P10605
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CTSB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 91 | |||||
| Range % | 83-95 | ||||||
| 1:2 | Average % | 98 | |||||
| Range % | 92-103 | ||||||
| 1:4 | Average % | 96 | |||||
| Range % | 85-100 | ||||||
| 1:8 | Average % | 103 | |||||
| Range % | 92-107 | ||||||
回收率：
| The recovery of mouse CTSB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 94 | 91-97 | |||||
| EDTA plasma (n=4) | 100 | 92-104 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected10002.6282.5322.5802.4745002.3152.1892.2522.1462501.8561.7761.8161.7101251.1241.1021.1131.00762.50.7580.7340.7460.64031.20.4720.4630.4680.36215.60.3150.3020.3090.20300.1040.1080.106 | |||||||
货期：3-5 working days
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