| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTGES3 |
| Uniprot No | Q15185 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-160aa |
| 氨基酸序列 | MQPASAKWYDRRDYVFIEFCVEDSKDVNVNFEKSKLTFSCLGGSDNFKHLNEIDLFHCIDPNDSKHKRTDRSILCCLRKGESGQSWPRLTKERAKLNWLSVDFNNWKDWEDDSDEDMSNFDRFSEMMNNMGGDEDVDLPEVDGADDDSQDSDDEKMPDLE |
| 预测分子量 | 20.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PTGES3重组蛋白的示例参考文献(注：文献为模拟示例，非真实存在)：
---
1. **Title**: "Recombinant PTGES3 Expression in Escherichia coli: Structural Insights and Functional Characterization"
**Authors**: Zhang L, et al.
**Summary**: 本研究在大肠杆菌中成功表达并纯化了重组PTGES3蛋白，通过X射线晶体学解析其三维结构，揭示了其催化活性位点的关键氨基酸残基，并验证其体外催化前列腺素E2合成的酶活性。
2. **Title**: "PTGES3 Interaction with Heat Shock Protein 90 (HSP90): A Recombinant Protein-Based Study"
**Authors**: Kim S, et al.
**Summary**: 利用重组PTGES3蛋白进行pull-down实验和表面等离子体共振(SPR)分析，证实PTGES3通过与HSP90形成复合物调控其稳定性，并影响肿瘤细胞中炎症相关信号通路。
3. **Title**: "Functional Role of Recombinant PTGES3 in Cancer Cell Proliferation"
**Authors**: Patel R, et al.
**Summary**: 在哺乳动物细胞中过表达重组PTGES3.发现其通过上调COX-2/PGE2轴促进肺癌细胞增殖，提示PTGES3可能成为癌症治疗的潜在靶点。
---
建议通过PubMed或Web of Science搜索关键词“PTGES3 recombinant protein”或“PTGES3 expression”获取真实文献。
**Background of PTGES3 Recombinant Protein**
PTGES3 (Prostaglandin E Synthase 3), also known as cytosolic prostaglandin E2 synthase (cPGES), is a multifunctional protein encoded by the *PTGES3* gene in humans. It belongs to the glutathione-dependent prostaglandin E synthase family but exhibits unique dual functionality. Unlike other prostaglandin synthases, PTGES3 primarily acts as a molecular chaperone, interacting with heat shock protein 90 (HSP90) to stabilize client proteins, including steroid hormone receptors and kinases, thereby regulating cellular stress responses and signaling pathways. Secondarily, PTGES3 catalyzes the isomerization of prostaglandin H2 (PGH2) to prostaglandin E2 (PGE2), a lipid mediator involved in inflammation, pain, and fever.
Recombinant PTGES3 protein is engineered using heterologous expression systems, such as *E. coli* or mammalian cells, to produce highly purified, functional protein for research. Its recombinant form enables studies on its chaperone activity, enzymatic kinetics, and interactions with HSP90 or other binding partners. Researchers utilize PTGES3 recombinant protein to investigate its role in diseases like cancer, where its overexpression correlates with tumor progression, immune evasion, and chemotherapy resistance. It is also implicated in neurodegenerative disorders and chronic inflammatory conditions due to its regulatory effects on PGE2 synthesis and protein-folding pathways.
The development of PTGES3 recombinant protein has facilitated drug discovery, structural studies, and mechanistic insights into its dual roles, highlighting its potential as a therapeutic target for inflammation-related pathologies and malignancies.
×
