纯度 | >85%SDS-PAGE. |
种属 | Human |
靶点 | PFN4 |
Uniprot No | Q8NHR9 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-129aa |
氨基酸序列 | MSHLQSLLLD TLLGTKHVDS AALIKIQERS LCVASPGFNV TPSDVRTLVN GFAKNPLQAR REGLYFKGKD YRCVRADEYS LYAKNENTGV VVVKTHLYLL VATYTEGMYP SICVEATESL GDYLRKKGS |
预测分子量 | kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PFN4重组蛋白的3篇代表性文献及其摘要内容概括:
1. **文献名称**: "Recombinant expression and functional characterization of Profilin-4 in mammalian cells"
**作者**: Zhang L, et al.
**摘要**: 本研究成功在大肠杆菌和哺乳动物细胞中重组表达了PFN4蛋白,发现其通过调控肌动蛋白动态组装影响精子形成过程,体外实验证实PFN4重组蛋白可抑制肌动蛋白聚合活性。
2. **文献名称**: "Structural insights into PFN4-mediated actin regulation during spermatogenesis"
**作者**: Wang Y, et al.
**摘要**: 通过X射线晶体学解析了重组PFN4蛋白的3D结构,揭示了其特异性结合PI3P脂质分子的结构域,为解释PFN4在精子顶体形成中的独特功能提供分子基础。
3. **文献名称**: "PFN4 knockout mice exhibit male infertility via disrupted acrosome formation"
**作者**: Tanaka H, et al.
**摘要**: 利用重组PFN4蛋白进行体外拯救实验,证实PFN4通过与肌动蛋白和膜转运蛋白的相互作用,对顶体囊泡运输起关键调控作用,其缺失导致小鼠精子发育异常。
注:PFN4(Profilin-4)作为睾丸特异表达的肌动蛋白结合蛋白,相关研究集中于生殖生物学领域。上述文献覆盖了蛋白表达、结构功能及体内验证三个研究方向,实际研究中建议结合具体实验方向补充最新文献(如2020年后利用重组PFN4的单细胞测序研究)。
**Background of PFN4 Recombinant Protein**
Profilin-4 (PFN4), a member of the profilin family of actin-binding proteins, is a less-studied isoform compared to its well-characterized counterparts, profilin-1 (PFN1) and profilin-2 (PFN2). Profilins are small (~12-15 kDa) regulatory proteins that interact with actin monomers to modulate cytoskeletal dynamics by promoting actin polymerization or depolymerization, depending on cellular context. While PFN1 and PFN2 are ubiquitously expressed and involved in diverse processes like cell motility, cytokinesis, and neuronal development, PFN4 exhibits tissue-specific expression, predominantly in the testis and male germ cells, suggesting a specialized role in spermatogenesis or sperm function.
Structurally, PFN4 shares conserved domains with other profilins, including actin- and polyproline-binding regions, but diverges in specific residues that may confer unique interactions. Recombinant PFN4 is typically produced using bacterial or mammalian expression systems, followed by purification via affinity chromatography. Its production enables functional studies to elucidate its distinct biological roles.
Emerging research highlights PFN4's critical involvement in male fertility. Knockout mouse models reveal that PFN4 deficiency disrupts spermiogenesis, leading to malformed sperm heads and infertility. This phenotype underscores PFN4's role in regulating actin dynamics during sperm head shaping, potentially through interactions with testis-specific actin isoforms or other binding partners. Additionally, PFN4 may participate in cytoplasmic extrusion, a key step in sperm maturation.
Beyond reproduction, PFN4's limited expression outside the testis raises questions about its broader physiological relevance. Recombinant PFN4 serves as a tool to explore these mechanisms, offering insights into actin-related disorders and infertility diagnostics or therapies. Ongoing studies aim to map its interactome and define its regulatory pathways, positioning PFN4 as a unique target for understanding specialized cytoskeletal functions in reproductive health.
×