首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠C-X-C趋化因子14（CXCL14）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的CXCL14含量。CXCL14是一种非ELR CXC趋化因子，主要对单核细胞具有趋化活性，参与癌症、免疫反应和上皮细胞增殖迁移。研究显示，CXCL14在结直肠癌细胞中表达与肝转移相关，可能通过增强血管内皮细胞增殖与迁移能力来增加血管新生，从而促进结直肠癌的肝转移。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于探索CXCL14在免疫性疾病模型、肿瘤微环境动态变化、代谢调控网络中的表达规律及其分子机制研究；可为探索CXCL14在细胞信号传导、宿主 - 病原体互作或疾病动物模型中的功能提供可靠检测工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cxcl14 ELISA Kit; Bmac ELISA Kit; Kec ELISA Kit; Ks1 ELISA Kit; Mip2g ELISA Kit; Scyb14C-X-C motif chemokine 14 ELISA Kit; B-cell and monocyte-activating chemokine ELISA Kit; Chemokine BRAK ELISA Kit; Kidney-expressed chemokine CXC ELISA Kit; MIP-2G ELISA Kit; Small-inducible cytokine B14 ELISA Kit
缩写：CXCL14
Uniprot No.：Q9WUQ5
种属：Mus musculus (Mouse)
样本类型：serum, plasma
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CXCL14 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 84 | |||||
| Range % | 80-92 | ||||||
| 1:2 | Average % | 103 | |||||
| Range % | 99-108 | ||||||
| 1:4 | Average % | 97 | |||||
| Range % | 92-101 | ||||||
| 1:8 | Average % | 89 | |||||
| Range % | 84-93 | ||||||
回收率：
| The recovery of mouse CXCL14 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 90-100 | |||||
| EDTA plasma (n=4) | 97 | 92-101 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected202.7372.6522.6952.547102.2352.2762.2562.10851.5331.6041.5691.4212.50.9150.9380.9270.7791.250.5570.5240.5410.3930.6250.3120.3270.3200.1720.3120.2390.2290.2340.08600.1460.1500.148 | |||||||
货期：3-5 working days
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