| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | SRPS2; SRPS2A; NY-REN-55 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human NEK1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NEK1抗体的3篇参考文献摘要示例(注：以下内容为基于领域知识的模拟描述，实际文献需通过学术数据库查询)：
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1. **文献名称**：*NEK1 mutations in familial and sporadic amyotrophic lateral sclerosis*
**作者**：Kenyan Project et al.
**摘要**：该研究通过全基因组测序发现NEK1基因突变与家族性和散发性肌萎缩侧索硬化症(ALS)相关。研究使用NEK1抗体进行蛋白表达水平分析，发现突变导致NEK1功能缺失，影响神经元DNA修复和微管稳定性。
2. **文献名称**：*Functional characterization of NEK1 kinase activity in DNA damage response*
**作者**：Hughes S, et al.
**摘要**：作者利用NEK1特异性抗体进行免疫沉淀和免疫荧光实验，证明NEK1通过磷酸化下游靶点(如HSP90)参与DNA损伤修复通路，并揭示其在维持基因组稳定性中的关键作用。
3. **文献名称**：*Development and validation of a polyclonal NEK1 antibody for subcellular localization studies*
**作者**：Meek DW, et al.
**摘要**：本研究开发了一种高特异性兔源多克隆NEK1抗体，并通过Western blot和免疫组化验证其在细胞系和组织样本中的应用。结果显示NEK1主要定位于细胞核及中心体，提示其在细胞周期调控中的潜在功能。
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如需具体文献，建议通过PubMed或Google Scholar搜索关键词“NEK1 antibody”、“NEK1 kinase function”或结合研究领域(如ALS、DNA repair)进一步筛选。
NEK1 (NIMA-related kinase 1) is a serine/threonine kinase involved in diverse cellular processes, including cell cycle regulation, DNA damage response, microtubule dynamics, and cilia formation. It is encoded by the *NEK1* gene, and mutations in this gene have been linked to human diseases, notably amyotrophic lateral sclerosis (ALS) and short-rib thoracic dysplasia. NEK1 dysfunction is particularly associated with ALS pathogenesis, where homozygous mutations or reduced expression disrupt DNA repair and neuronal homeostasis.
NEK1 antibodies are essential tools for studying the protein's expression, localization, and functional roles. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence to detect NEK1 in cell lines, tissues, or animal models. Commercially available antibodies target specific epitopes, such as the N-terminal or kinase domains, enabling researchers to investigate NEK1’s post-translational modifications or interaction partners.
Research utilizing NEK1 antibodies has advanced understanding of its dual role in health and disease. For instance, studies reveal NEK1’s involvement in the DNA damage response through interactions with proteins like ATM/ATR, and its regulatory effects on primary cilia in developmental disorders. In ALS, NEK1 antibody-based assays help identify pathogenic variants and assess therapeutic strategies targeting kinase activity or protein stability. However, challenges remain in antibody specificity and standardization across experimental models, necessitating rigorous validation. Overall, NEK1 antibodies remain pivotal in elucidating molecular mechanisms and potential therapeutic targets for NEK1-related diseases.
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