| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | HCA4; LICS; NSE3; NDNL2; MAGEG1; MAGEL3 |
| WB Predicted band size | 34 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human NSMCE3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NSMCE3抗体的3篇参考文献示例(注：部分内容为模拟概括，仅供参考)：
1. **文献名称**：*The SMC5/6 Complex Requires NSMCE3 to Ubiquitinate DNA Repair Proteins*
**作者**：Li et al., 2020
**摘要**：本研究揭示了NSMCE3作为SMC5/6复合体的关键亚基，通过其E3泛素连接酶活性调控DNA损伤修复。作者利用NSMCE3特异性抗体，验证了其在复合体中的定位及对BRCA1泛素化的调控作用。
2. **文献名称**：*NSMCE3 Deficiency Leads to Genomic Instability and Enhanced Cancer Susceptibility*
**作者**：Garcia-Exposito et al., 2018
**摘要**：通过构建NSMCE3缺陷小鼠模型，结合免疫印迹(使用NSMCE3抗体)和染色体分析，证明NSMCE3缺失导致DNA复制应激加剧，显著增加淋巴瘤发病率。
3. **文献名称**：*A Structural Analysis of the SMC5/6 Complex in Chromosome Segregation*
**作者**：Woo et al., 2019
**摘要**：通过冷冻电镜和NSMCE3抗体的免疫共沉淀实验，解析了SMC5/6复合体的三维结构，阐明NSMCE3在维持复合体稳定性及染色体分离中的关键作用。
4. **文献名称**：*NSMCE3 Antibody Development for Diagnostic Applications in Breast Cancer*
**作者**：Chen et al., 2021
**摘要**：研究团队开发了一种高特异性NSMCE3单克隆抗体，用于乳腺癌组织样本检测，发现NSMCE3低表达与患者预后不良显著相关，提示其作为潜在生物标志物的价值。
**备注**：以上文献为示例性内容，实际研究中请通过学术数据库(如PubMed、Web of Science)检索最新及具体文献。若需真实文献，建议以“NSMCE3 antibody”“SMC5-SMC6 complex”等关键词进行精准检索。
The NSMCE3 antibody is a research tool designed to target the NSMCE3 protein (also known as SMC5 structural maintenance of chromosomes 5-6 complex subunit E3), a critical component of the SMC5/6 complex. This highly conserved protein complex plays essential roles in maintaining genomic stability, including DNA repair, replication fork restart, and chromosome segregation during cell division. NSMCE3. along with other subunits, contributes to the structural and functional integrity of the SMC5/6 complex, which is particularly vital for resolving DNA recombination intermediates and preventing aberrant chromosomal rearrangements.
Antibodies against NSMCE3 are widely used in molecular biology to study its expression, localization, and interactions within the SMC5/6 complex. They enable techniques like Western blotting, immunofluorescence, and immunoprecipitation to explore NSMCE3's role in DNA damage response pathways and its potential links to diseases such as cancer or genetic disorders. Dysregulation of SMC5/6 components has been implicated in genomic instability and tumorigenesis, making NSMCE3 antibodies valuable for investigating mechanisms underlying genome maintenance and developing targeted therapies. Researchers also utilize these antibodies to validate CRISPR/Cas9 knockout models or assess protein levels in clinical samples, aiding both basic research and translational studies.
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