首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠CXC趋化因子受体4(CXCR4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的CXCR4含量。CXCR4是一种G蛋白偶联受体，作为趋化因子CXCL12的受体，在细胞迁移、增殖和肿瘤生长中发挥关键作用。其研究机制涉及CXCL12与CXCR4的相互作用，激活下游信号通路，如AKT信号传导，影响细胞迁移和肿瘤微环境。CXCR4在多种癌症中作为治疗靶点具有潜在价值。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品适用于科研领域中对CXCR4相关生物学过程的研究，如肿瘤微环境分析、免疫细胞趋化机制探讨、炎症性疾病模型构建等，为体外研究CXCR4在病理生理过程中的作用及药物干预效果评估提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cxcr4; Cmkar4; C-X-C chemokine receptor type 4; CXC-R4; CXCR-4; Fusin; Leukocyte-derived seven transmembrane domain receptor; LESTR; Stromal cell-derived factor 1 receptor; SDF-1 receptor; CD antigen CD184
缩写：CXCR4
Uniprot No.：O08565
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CXCR4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 103 | ||||
| Range % | 97-106 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 85-96 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-100 | |||||
回收率：
| The recovery of rat CXCR4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 96 | 92-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected101.8681.8561.8621.77551.1331.1221.1281.0412.50.7420.7570.7500.6631.250.5480.5320.5400.4530.6250.3260.3120.3190.2320.3120.2240.2180.2210.1340.1560.1740.1850.1800.09300.0880.0860.087 | ||||||
货期：3-5 working days
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