首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人NADH-细胞色素b5还原酶3(CYB5R3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CYB5R3含量。CYB5R3是重要靶点。它参与体内多种生物化学反应，如脂肪酸代谢等。研究机制方面，主要聚焦其在电子传递链中的作用，明确其对维持细胞内环境稳定意义重大。其功能异常和一些疾病相关，目前正深入研究为疾病治疗提供方向。试剂盒检测范围为25 pg/mL-1600 pg/mL，适用于研究CYB5R3在氧化应激反应、脂质代谢调控中的分子机制，或作为药物开发中代谢通路研究的检测工具，亦可用于遗传代谢疾病模型构建时的生物标志物分析。为血红素代谢通路研究、药物毒性评估及疾病机制探索提供灵敏的定量检测方案，满足细胞生物学、药理学等领域的科研需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：B5R ELISA Kit; Cyb5r3 ELISA Kit; Cytochrome b5 reductase 3 ELISA Kit; Cytochrome b5 reductase ELISA Kit; DIA1 ELISA Kit; Diaphorase 1 ELISA Kit; Diaphorase-1 ELISA Kit; NADH cytochrome b5 reductase 3 ELISA Kit; NADH-cytochrome b5 reductase 3 membrane-bound form ELISA Kit; NADH-cytochrome b5 reductase 3 soluble form ELISA Kit; NB5R3_HUMAN ELISA Kit; OTTHUMP00000028761 ELISA Kit; OTTHUMP00000198435 ELISA Kit; OTTHUMP00000198574 ELISA Kit; OTTHUMP00000198662 ELISA Kit; OTTHUMP00000198665 ELISA Kit
缩写：CYB5R3
Uniprot No.：P00387
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CYB5R3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 105 | |||||
| Range % | 94-110 | ||||||
| 1:2 | Average % | 104 | |||||
| Range % | 97-109 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 83-99 | ||||||
| 1:8 | Average % | 90 | |||||
| Range % | 83-93 | ||||||
回收率：
| The recovery of human CYB5R3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 84-96 | |||||
| EDTA plasma (n=4) | 107 | 100-111 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected16002.3232.2632.2932.1838001.7231.6491.6861.5764001.2081.1581.1831.0732000.6420.6660.6540.5441000.4230.4170.4200.310500.2700.2640.2670.157250.1850.1790.1820.07200.1080.1120.110 | |||||||
货期：3-5 working days
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