| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | HTR1C; 5-HT2C; 5HTR2C; 5-HTR2C |
| WB Predicted band size | 52 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human HTR2C |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HTR2C抗体的3篇参考文献示例(注：以下内容为模拟示例，实际文献需根据数据库检索确认)：
1. **文献名称**：*"Selective monoclonal antibody for serotonin 5-HT2C receptor labeling in mammalian brain"*
**作者**：Smith, J.K. et al.
**摘要**：本研究开发了一种高特异性的单克隆抗体，用于识别哺乳动物脑组织中的HTR2C受体。通过免疫组化和Western blot验证，该抗体在啮齿类及人类脑切片中显示出低交叉反应性，适用于神经解剖学研究。
2. **文献名称**：*"Validation of a novel anti-5-HT2C antibody for functional studies in psychiatric disorders"*
**作者**：Lee, M.T. & Garcia, R.
**摘要**：文章验证了一种新型HTR2C抗体的功能应用，证明其可用于流式细胞术和免疫沉淀。研究进一步利用该抗体发现抑郁症模型中HTR2C表达水平的变化，提示其作为治疗靶点的潜力。
3. **文献名称**：*"Comparative analysis of commercial 5-HT2C receptor antibodies reveals variability in specificity and sensitivity"*
**作者**：Zhang, Y. et al.
**摘要**：通过比较市售的5种HTR2C抗体，研究发现不同抗体的特异性和灵敏度差异显著，部分抗体存在与非靶标受体(如HTR2A)的交叉反应，强调了抗体选择对实验结果可靠性的重要性。
4. **文献名称**：*"5-HT2C receptor antibody modulates feeding behavior in zebrafish via hypothalamic signaling"*
**作者**：Brown, S.E. et al.
**摘要**：利用HTR2C特异性抗体阻断斑马鱼下丘脑受体活性，研究发现其可显著减少摄食行为，揭示了HTR2C在能量平衡调控中的进化保守机制。
建议通过PubMed或Google Scholar以关键词“HTR2C antibody”、“5-HT2C receptor antibody”检索最新文献以获取真实数据。
The HTR2C antibody is a research tool designed to target the 5-hydroxytryptamine receptor 2C (HTR2C), a G protein-coupled receptor (GPCR) primarily activated by serotonin. HTR2C is widely expressed in the central nervous system, particularly in regions like the choroid plexus, hypothalamus, and hippocampus, where it modulates mood, appetite, sleep, and cognitive functions. Dysregulation of HTR2C signaling has been implicated in neuropsychiatric disorders, including depression, anxiety, schizophrenia, and obesity.
HTR2C antibodies are critical for studying receptor localization, expression levels, and functional interactions in tissues or cell models. They are commonly used in techniques such as Western blotting, immunohistochemistry (IHC), and flow cytometry. Many commercially available HTR2C antibodies are raised against specific epitopes, often within extracellular or intracellular domains, and validated for reactivity in human, rat, or mouse samples. However, challenges persist due to high sequence homology (~50-80%) among 5-HT2 receptor subtypes (HTR2A, HTR2B, HTR2C), necessitating rigorous validation via knockout controls or orthogonal methods (e.g., CRISPR/Cas9-edited cells) to confirm specificity.
Recent studies emphasize HTR2C's role in drug development, particularly for obesity (e.g., lorcaserin, a former HTR2C agonist) and mental health therapies. Antibodies thus serve as essential tools to dissect receptor dynamics, ligand binding mechanisms, and downstream signaling pathways in both physiological and disease contexts.
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