Rabbit Polyclonal ASF1A Antibody

The ASF1A (Anti-silencing Function 1A) antibody is a tool used to detect and study the ASF1A protein, a conserved histone chaperone critical for chromatin assembly and disassembly during DNA replication, repair, and transcription. ASF1A, along with its paralog ASF1B, facilitates the deposition of histones H3/H4 onto newly synthesized DNA, ensuring proper nucleosome formation and epigenetic inheritance. It interacts with key regulators like HIRA and CAF-1 complexes, playing roles in replication-coupled nucleosome assembly, transcriptional silencing, and genome stability. Dysregulation of ASF1A is linked to cancer progression, as its overexpression is observed in tumors and correlates with poor prognosis. Researchers use ASF1A antibodies in techniques like Western blotting, immunofluorescence, and ChIP to investigate its expression, localization, and interactions in cellular processes. These antibodies help elucidate ASF1A's involvement in replication stress response, chromatin remodeling, and DNA damage repair pathways. Specificity validation (e.g., via knockout controls) is essential due to structural similarities between ASF1A and ASF1B. Studies employing ASF1A antibodies have advanced understanding of its dual roles in promoting tumorigenesis through cell cycle regulation and suppressing genomic instability.