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产品描述：人细胞角蛋白21-1片段(CYFRA21-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的CYFRA21-1含量。CYFRA21-1是一种细胞角蛋白片段，广泛分布于正常组织中。当细胞癌变时，角蛋白被降解并释放到血液中，CYFRA21-1水平升高。它作为肺癌，尤其是鳞状细胞癌的标志物，可用于诊断和预后评估。试剂盒检测范围为0.312 ng/mL-20 ng/mL，本产品适用于基础医学研究中肿瘤标志物表达水平检测、疾病机制探索或药物开发过程中的生物标志物动态监测，为体外实验提供血清/血浆样本中CYFRA21 - 1浓度的可靠数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：CYFRA21-1
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CYFRA21-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 82-90 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 94-110 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 86-99 | |||||
回收率：
| The recovery of human CYFRA21-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 80-98 | ||||
| EDTA plasma (n=4) | 97 | 90-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.5412.3852.4632.305102.2292.0822.1561.99851.6071.5451.5761.4182.50.8660.8420.8540.6961.250.4630.4390.4510.2930.6250.3370.3240.3310.1730.3120.2540.2460.2500.09200.1600.1560.158 | ||||||
货期：3-5 working days
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