首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：人细胞色素C(Cyt-C)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、细胞裂解物样本中的CYCS含量。CYCS（细胞色素c）是一种位于线粒体内膜上的电子传递蛋白，参与细胞呼吸链和细胞凋亡过程。其功能包括在线粒体电子传递链中传递电子，以及在细胞凋亡时从线粒体释放到细胞质中，激活caspase-9并引发凋亡。研究CYCS有助于理解细胞能量代谢和程序性死亡调控。试剂盒检测范围为7.8 ng/mL-500 ng/mL，该产品主要应用于细胞凋亡机制研究、药物诱导的线粒体功能评估、代谢性疾病模型建立等科研领域，例如在体外培养细胞模型中监测凋亡进程，或通过检测血清/血浆样本探索疾病相关生物标志物。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CYC ELISA Kit; CYC_HUMAN ELISA Kit; CYCS ELISA Kit; Cytochrome c ELISA Kit; Cytochrome c somatic ELISA Kit; HCS ELISA Kit; THC4 ELISA Kit
缩写：CYCS
Uniprot No.：P99999
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell lysates
检测范围：7.8 ng/mL-500 ng/mL
灵敏度：1.95 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Cyt-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 82-105 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 85-100 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 92-107 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 83-98 | |||||
回收率：
| The recovery of human Cyt-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-99 | ||||
| EDTA plasma (n=4) | 95 | 88-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected5002.3372.4042.3712.2222501.6921.6591.6761.5271251.1061.1381.1220.97362.50.6750.6620.6690.52031.20.4360.4280.4320.28315.60.2570.2730.2650.1167.80.2060.2150.2110.06200.1530.1450.149 | ||||||
货期：3-5 working days
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