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产品描述：人细胞色素C - 1(CYC1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CYC1含量。CYC1是重要的细胞色素c1蛋白编码基因，在细胞呼吸链电子传递中发挥关键作用。研究主要围绕其在能量代谢中的功能机制，以及其异常与肿瘤、神经退行性疾病等的关联展开，以探寻新的疾病诊断标志物和治疗靶点。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于探索心血管疾病、神经退行性疾病或癌症模型中CYC1的生物学功能及动态变化，可为细胞代谢研究、药物作用机制分析或疾病相关分子通路探索提供可靠数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Complex III subunit 4 ELISA Kit; Complex III subunit IV ELISA Kit; CY1_HUMAN ELISA Kit; CYC1 ELISA Kit; Cytochrome b c1 complex subunit 4 ELISA Kit; Cytochrome b-c1 complex subunit 4 ELISA Kit; Cytochrome bc1 complex subunit 4 ELISA Kit; Cytochrome c 1 ELISA Kit; Cytochrome c-1 ELISA Kit; Cytochrome c1; heme protein; mitochondrial ELISA Kit; Ubiquinol cytochrome c reductase complex cytochrome c1 subunit ELISA Kit; Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit ELISA Kit; UQCR4 ELISA Kit
缩写：CYC1
Uniprot No.：P08574
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of Human CYC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 85-93 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 93-101 | |||||
回收率：
| The recovery of Human CYC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 102 | 97-106 | ||||
| EDTA plasma (n=4) | 87 | 83-91 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.8632.9672.9152.81710002.5262.4252.4762.3785001.8751.7761.8261.7282501.0651.0431.0540.9561250.5470.5240.5360.43862.50.2840.2720.2780.18031.250.1980.1890.1940.09600.0970.0980.098 | ||||||
货期：3-5 working days
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