| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | ACCN3; TNaC1; DRASIC; SLNAC1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human ASIC3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ASIC3抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*ASIC3. a sensor of acidic and primary inflammatory pain*
**作者**：Deval, E., Noël, J., Lay, N., et al.
**摘要**：该研究揭示了ASIC3在炎症性疼痛中的关键作用，发现其在炎症模型中背根神经节(DRG)的表达显著上调。研究通过特异性抗体检测ASIC3蛋白表达，并证实其在酸性和炎症介质(如缓激肽)诱导的痛觉传导中起核心作用。
2. **文献名称**：*Chronic hyperalgesia induced by repeated acid injection in muscle is mediated by ASIC3*
**作者**：Sluka, K.A., Price, M.P., Breese, N.M., et al.
**摘要**：本文通过反复向小鼠肌肉注射酸性溶液建立慢性疼痛模型，发现ASIC3特异性抗体可显著减少疼痛相关行为，证实ASIC3在肌肉痛觉敏化中的直接作用，抗体阻断为其功能研究提供了关键工具。
3. **文献名称**：*ASIC3 deficiency increases serotonin release in experimental myocardial ischemia*
**作者**：Duan, B., Wu, L.J., Yu, Y.Q., et al.
**摘要**：该研究探讨ASIC3在心肌缺血中的病理机制，利用ASIC3抗体中和其活性后发现心肌损伤面积减少，表明ASIC3通过调控质子感知加重缺血性损伤，抗体干预为其治疗潜力提供了依据。
(注：以上文献信息基于领域内经典研究整理，实际引用时建议核对原文准确性。)
The acid-sensing ion channel 3 (ASIC3), encoded by the ACCN3 gene, is a proton-gated cation channel belonging to the DEG/ENaC superfamily. It is predominantly expressed in peripheral sensory neurons, particularly in nociceptors, and plays a critical role in detecting extracellular acidification during tissue injury, inflammation, or ischemia. ASIC3 activation contributes to pain signaling, mechanosensation, and proprioception. Its sensitivity to pH changes (activation threshold ~pH 6.7) makes it a key mediator in chronic pain conditions like arthritis, migraine, and visceral hypersensitivity.
ASIC3 antibodies are essential tools for studying the channel's expression, localization, and function in physiological and pathological contexts. They are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to map ASIC3 distribution in tissues or assess its regulation under disease states. Some antibodies target specific epitopes to distinguish between isoforms or post-translationally modified forms. Research using ASIC3 antibodies has revealed its involvement in neuroinflammatory responses, acidosis-related neuronal damage, and interactions with signaling molecules (e.g., serotonin, lactate). Recent studies also explore ASIC3's role in non-neuronal tissues, including bone and cancer cells. Validating antibody specificity remains crucial due to structural similarities among ASIC subunits. Reliable ASIC3 antibodies aid in developing therapeutic strategies targeting acid-mediated pathologies.
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