| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | 5-HT3B |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human HTR3B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HTR3B抗体的示例参考文献(注：以下内容为模拟示例，非真实文献)：
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1. **标题**: *Development and Characterization of a Novel Monoclonal Antibody Targeting the HTR3B Receptor Subunit*
**作者**: Smith A, et al.
**摘要**: 本研究开发了一种特异性靶向HTR3B亚基的单克隆抗体，验证了其在细胞系和脑组织中的结合能力。该抗体可用于研究HTR3B在神经精神疾病(如焦虑症和抑郁症)中的表达模式及功能调控。
2. **标题**: *HTR3B Antibodies as Biomarkers in Irritable Bowel Syndrome*
**作者**: Chen L, et al.
**摘要**: 通过分析肠易激综合征(IBS)患者的血清样本，发现HTR3B自身抗体的水平与疾病严重程度相关，提示其可能作为IBS的新型诊断标志物或治疗靶点。
3. **标题**: *Structural Insights into HTR3B-Antibody Interactions via Cryo-EM*
**作者**: Tanaka K, et al.
**摘要**: 利用冷冻电镜技术解析了HTR3B与其特异性抗体的复合物结构，揭示了抗体结合表位对受体通道活性的抑制作用，为靶向药物设计提供了结构基础。
4. **标题**: *Role of Anti-HTR3B Autoantibodies in Chemotherapy-Induced Nausea*
**作者**: Müller S, et al.
**摘要**: 研究发现部分接受化疗的患者体内存在HTR3B自身抗体，这些抗体可能通过干扰5-HT3受体功能减轻化疗相关性恶心，为个体化止吐治疗提供了新思路。
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**说明**：以上文献为虚构示例，实际研究中建议通过PubMed或Web of Science等平台检索关键词(如“HTR3B antibody”“5-HT3B receptor autoantibody”)获取真实文献。
The HTR3B antibody targets the 5-hydroxytryptamine receptor 3B (HTR3B), a subunit of the serotonin-gated ion channel receptor family (5-HT3 receptor). This receptor, primarily expressed in the central and peripheral nervous systems, mediates fast synaptic transmission by allowing cation influx upon serotonin binding. The 5-HT3 receptor exists as a pentamer, with HTR3B often combining with HTR3A subunits to form heteromeric channels. These receptors play critical roles in regulating emesis, pain perception, and gastrointestinal function, making them targets for anti-nausea drugs (e.g., ondansetron) in chemotherapy.
HTR3B-specific antibodies are essential tools for studying receptor distribution, subunit composition, and functional differences between homomeric (HTR3A-only) and heteromeric (HTR3A/HTR3B) receptors. They enable techniques like immunohistochemistry, Western blotting, and flow cytometry to explore tissue-specific expression patterns or disease associations. Dysregulation of HTR3B has been implicated in irritable bowel syndrome, psychiatric disorders, and chemotherapy-induced vomiting. Research using these antibodies also investigates genetic polymorphisms (e.g., HTR3B p.Tyr129Ser) linked to variable drug responses.
Commercial HTR3B antibodies are typically validated in knockout models to ensure subunit specificity, given the structural similarity among 5-HT3 receptor subunits. Their applications span basic neuroscience, drug development, and clinical biomarker studies.
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