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产品描述：人1,25-二羟维生素D(3)24-羟化酶，线粒体(CYP24A1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CYP24A1含量。CYP24A1是一个重要靶点。它在维生素D代谢中发挥关键作用，参与调节体内维生素D水平的稳态。研究机制方面，聚焦于其对维生素D的羟化作用及相关信号通路。抑制CYP24A1有望用于治疗多种疾病，如癌症、骨质疏松等。试剂盒检测范围为7.8 pg/mL-500 pg/mL，适用于体外研究维生素D代谢调控机制、评估药物干预对酶活性影响，或探索慢性肾病、佝偻病、恶性肿瘤等疾病模型中该酶的动态变化。支持科研人员高效开展细胞、动物或临床样本的维生素D代谢通路研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：1 25 @dihydroxyvitamin D3 24 hydroxylase ELISA Kit; 1 25 dihydroxyvitamin D(3) 24 hydroxylase ELISA Kit; 1 ELISA Kit; 1,25-dihydroxyvitamin D(3) 24-hydroxylase; mitochondrial ELISA Kit; 24 OHase ELISA Kit; 24-OHase ELISA Kit; 25-dihydroxyvitamin D(3) 24-hydroxylase ELISA Kit; CP 24 ELISA Kit; CP24 ELISA Kit; CP24A_HUMAN ELISA Kit; CYP 24 ELISA Kit; CYP24 ELISA Kit; CYP24A1 ELISA Kit; Cytochrome P450 24A1 ELISA Kit; Cytochrome P450 24A1 mitochondrial ELISA Kit; cytochrome P450 CC24 ELISA Kit; Cytochrome P450 family 24 ELISA Kit; Cytochrome P450 family 24 subfamily A member 1 ELISA Kit; Cytochrome P450 family 24 subfamily A polypeptide 1 ELISA Kit; Cytochrome P450 subfamily XXIV ELISA Kit; Cytochrome P450; subfamily XXIV (vitamin D 24-hydroxylase) ELISA Kit; Cytochrome P450-CC24 ELISA Kit; EC 1.14.13.n4 ELISA Kit; Exo mitochondrial protein ELISA Kit; HCAI ELISA Kit; HCINF1 ELISA Kit; MGC126273 ELISA Kit; MGC126274 ELISA Kit; mitochondrial ELISA Kit; P450 CC24 ELISA Kit; Vitamin D 24 hydroxylase ELISA Kit; Vitamin D(3) 24 hydroxylase ELISA Kit; Vitamin D(3) 24-hydroxylase ELISA Kit
缩写：CYP24A1
Uniprot No.：Q07973
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CYP24A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 86-97 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 83-94 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 91-102 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 92-103 | |||||
回收率：
| The recovery of human CYP24A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 88-99 | ||||
| EDTA plasma (n=4) | 86 | 80-91 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.7192.6122.6662.5342502.2262.1212.1742.0421251.4071.5041.4561.32462.50.8450.8210.8330.70131.20.4570.4660.4620.33015.60.3230.3140.3190.1877.80.2210.2260.2240.09200.1330.1310.132 | ||||||
货期：3-5 working days
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