| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | ELG1; FRAG1; C17orf41 |
| WB Predicted band size | 207 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human ATAD5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ATAD5抗体的虚构参考文献示例(仅供示例用途，非真实文献)：
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1. **文献名称**：*ATAD5 Antibody Development for Detecting Genomic Instability in Human Cells*
**作者**：Smith J, et al.
**摘要**：本研究开发了一种高特异性的ATAD5多克隆抗体，用于检测人类细胞中ATAD5蛋白的表达水平。实验验证显示，该抗体可特异性识别ATAD5的C端结构域，并在免疫印迹和免疫荧光中有效标记ATAD5蛋白，为研究ATAD5在DNA复制后修复中的作用提供了工具。
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2. **文献名称**：*ATAD5 as a Biomarker in Ovarian Cancer: Antibody-Based Detection and Clinical Correlations*
**作者**：Lee H, et al.
**摘要**：通过ATAD5抗体的免疫组化分析，研究者在卵巢癌组织中发现ATAD5蛋白表达显著下调，并与患者预后不良相关。该研究揭示了ATAD5在维持基因组稳定性中的关键作用，并验证了抗体在临床样本中的诊断潜力。
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3. **文献名称**：*Functional Characterization of ATAD5-PCNA Interaction Using Monoclonal Antibodies*
**作者**：Zhang R, et al.
**摘要**：利用自主研发的ATAD5单克隆抗体，研究者证实ATAD5通过与PCNA(增殖细胞核抗原)的直接相互作用调控DNA复制叉的稳定性。抗体阻断实验进一步揭示了ATAD5在复制压力条件下维持基因组完整性的机制。
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4. **文献名称**：*ATAD5 Knockout Mouse Model and Antibody-Based Tissue Staining*
**作者**：Johnson M, et al.
**摘要**：本研究利用ATAD5特异性抗体分析ATAD5敲除小鼠模型，发现ATAD5缺失导致胚胎致死性及DNA损伤修复缺陷。抗体在组织切片中的染色结果明确了ATAD5在增殖活跃组织(如肠道和骨髓)中的高表达。
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注：以上文献为模拟生成，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实文献。
ATAD5 (ATPase family AAA domain-containing protein 5), also known as ELG1 in yeast, is a conserved protein critical for maintaining genomic stability during DNA replication. It belongs to the AAA+ ATPase family and functions as a key regulator of replication stress responses. ATAD5 forms a complex with the replication clamp loader (RFC) and plays a vital role in unloading proliferating cell nuclear antigen (PCNA) from chromatin after DNA synthesis. This process ensures proper recycling of PCNA, prevents replication fork stalling, and mitigates DNA damage accumulation.
Dysregulation of ATAD5 is linked to genomic instability and cancer. Studies show that ATAD5 acts as a tumor suppressor, with mutations or reduced expression observed in ovarian, endometrial, and colorectal cancers. Its role in replication stress management makes it a focus in cancer biology and therapeutic research.
ATAD5 antibodies are essential tools for studying DNA replication, repair mechanisms, and cancer-related pathways. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect ATAD5 expression, localization, and interactions. Researchers also utilize these antibodies to investigate ATAD5's dynamics during the cell cycle or its response to replication-blocking agents. Understanding ATAD5's functions through antibody-based assays contributes to insights into genome maintenance mechanisms and potential cancer biomarkers or therapeutic targets.
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