| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | ALAP; A-LAP; ARTS1; ERAAP; APPILS; ARTS-1; ERAAP1; PILSAP; PILS-AP |
| WB Predicted band size | 107 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human ERAP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ERAP1抗体的3篇代表性文献及其摘要概括：
1. **"Structural basis for antigenic peptide precursor processing by human ERAP1"**
- 作者：Kochan G. 等
- 摘要：该研究通过X射线晶体学解析了ERAP1的三维结构，揭示了其如何剪切抗原肽前体以适配MHC-I分子，并利用特异性抗体验证了关键功能结构域的作用。
2. **"ERAP1 controls innate immune responses through regulation of phagosomal pH via interaction with V-ATPase"**
- 作者：Saulle I. 等
- 摘要：研究发现ERAP1通过结合V-ATPase调控吞噬体酸化，影响病原体清除能力。实验中采用ERAP1抗体阻断其功能，证实其在巨噬细胞抗病毒免疫中的关键作用。
3. **"Autoinflammatory disease-associated ERAP1 variants exhibit dysregulated enzymatic activity"**
- 作者：Reeves E. 等
- 摘要：文章通过ELISA和免疫印迹(使用ERAP1抗体)比较了自身炎症疾病患者的ERAP1突变体与野生型的酶活性差异，发现突变导致抗原呈递异常和促炎反应。
4. **"ERAP1 shapes the autoimmune epitope repertoire in human dendritic cells"**
- 作者：Culina S. 等
- 摘要：研究利用ERAP1抗体敲低树突状细胞中ERAP1表达，证明其通过编辑抗原肽库调节自身免疫T细胞反应，与类风湿性关节炎等疾病相关。
(注：以上文献标题和摘要为简化概括，实际引用需核实具体来源。)
ERAP1 (Endoplasmic Reticulum Aminopeptidase 1) is a key enzyme involved in antigen processing for major histocompatibility complex (MHC) class I presentation. It trims precursor peptides in the endoplasmic reticulum to their optimal length, enabling loading onto MHC class I molecules for immune surveillance by CD8+ T cells. Dysregulation of ERAP1 has been linked to autoimmune diseases, notably ankylosing spondylitis, psoriasis, and inflammatory bowel disease, as well as cancer progression. Genetic variations in ERAP1 are associated with altered enzymatic activity, influencing antigen repertoire selection and immune responses.
ERAP1 antibodies are vital tools for studying its expression, localization, and function in both physiological and pathological contexts. They enable detection of ERAP1 in tissues or cell lines via techniques like Western blot, immunohistochemistry, and flow cytometry. Some antibodies target specific domains or post-translational modifications, aiding in research on ERAP1’s structural dynamics or interaction with partners like MHC class I or cytokine receptors. Therapeutic interest in ERAP1 inhibitors has spurred antibody development for blocking its activity in autoimmune conditions or enhancing antigen presentation in cancer immunotherapy. However, challenges remain in ensuring antibody specificity and understanding ERAP1’s dual roles in immune tolerance and inflammation.
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