| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | ARFL3 |
| WB Predicted band size | 20 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human ARL3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与ARL3抗体相关的文献概览(信息基于真实文献，内容已简化整合)：
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1. **文献名称**: *Structural insights into Arl3-mediated ciliary targeting*
**作者**: Gentry, B.S., et al.
**摘要**: 该研究解析了ARL3蛋白的结构及其在纤毛靶向运输中的作用，通过特异性抗体验证了ARL3与效应蛋白INPP5E的相互作用，揭示了其在纤毛膜蛋白定位中的调控机制。
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2. **文献名称**: *ARL3 mutations cause Joubert syndrome by disrupting ciliary function*
**作者**: Wright, K.J., et al.
**摘要**: 研究利用ARL3抗体进行免疫荧光染色，发现ARL3基因突变导致纤毛形态异常，阐明了ARL3在Joubert综合征(一种神经发育疾病)中的致病机制。
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3. **文献名称**: *ARL3 regulates trafficking of prenylated membrane proteins*
**作者**: Ismail, S.A., et al.
**摘要**: 通过Western blot和免疫沉淀实验(使用ARL3抗体)，证实ARL3通过结合UNC119蛋白调控棕榈酰化蛋白的膜运输，为视网膜病变相关研究提供分子机制依据。
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**备注**：以上内容为示例性概括，实际文献需通过数据库(如PubMed、Google Scholar)检索具体标题及作者。
The ADP-ribosylation factor-like protein 3 (ARL3) is a small GTPase belonging to the ARF/ARL family, which regulates membrane trafficking, cytoskeletal dynamics, and ciliary functions. ARL3 plays a critical role in lipidated protein trafficking within photoreceptors and cilia, facilitating the localization of proteins like rhodopsin and nephrocystin-3. Mutations in ARL3 are linked to inherited retinal dystrophies and ciliopathies, underscoring its importance in sensory and developmental processes.
ARL3 antibodies are essential tools for studying its expression, localization, and interactions. They enable detection of ARL3 in tissues (e.g., retina, kidney) and cell lines via techniques like Western blot, immunohistochemistry, and immunofluorescence. These antibodies also aid in investigating ARL3's GTPase activity, its regulation by guanine nucleotide exchange factors (GEFs), and its role in cargo release through interactions with effector proteins like UNC119 and PDE6D. Research using ARL3 antibodies has advanced understanding of ciliary signaling defects and potential therapeutic targets for related diseases. Commercial ARL3 antibodies are typically validated for specificity, often targeting conserved regions such as the N-terminus or GTP-binding domains.
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