首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠CYR61蛋白(CYR61)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CYR61含量。CYR61是重要的细胞因子。其背景上参与细胞增殖、分化等多种生理过程。研究机制方面，它可与细胞膜上特定受体结合，激活下游信号通路，影响基因表达，在肿瘤发生、发展等病理过程中发挥作用，是潜在治疗靶点。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，科研人员可通过本产品研究CYR61在心血管疾病模型、肿瘤生长微环境或组织再生过程中的动态变化，尤其适用于分析病理状态下血管生成相关机制或细胞外基质蛋白相互作用研究。可为细胞实验、动物模型等基础研究提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ccn1 ELISA Kit; Cyr61 ELISA Kit; Igfbp10 ELISA Kit; CCN family member 1 ELISA Kit; 3CH61 ELISA Kit; Cellular communication network factor 1 ELISA Kit; Cysteine-rich angiogenic inducer 61 ELISA Kit; Insulin-like growth factor-binding protein 10 ELISA Kit; IBP-10 ELISA Kit; IGF-binding protein 10 ELISA Kit; IGFBP-10 ELISA Kit; Protein CYR61 ELISA Kit
缩写：CYR61
Uniprot No.：P18406
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：18.75 pg/mL-1200 pg/mL
灵敏度：4.68 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CYR61 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 91 | ||||
| Range % | 86-99 | |||||
| 1:200 | Average % | 97 | ||||
| Range % | 92-104 | |||||
| 1:400 | Average % | 93 | ||||
| Range % | 88-98 | |||||
| 1:800 | Average % | 88 | ||||
| Range % | 83-94 | |||||
回收率：
| The recovery of mouse CYR61 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 88-98 | ||||
| EDTA plasma (n=4) | 96 | 92-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 12002.5842.6522.6182.498 6002.1752.0752.1252.005 3001.6071.6031.6051.485 1501.0161.0901.0530.933 750.5030.5240.5140.394 37.50.3280.3550.3420.222 18.750.2320.2290.2310.111 00.1200.1190.120 | ||||||
货期：3-5 working days
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